摘要
为了探明锐顶镰孢菌对接种后苜蓿种苗的致病性,本研究采用菌饼接种法,将分离自4个地点的15个锐顶镰孢菌株接种于14个紫花苜蓿品种的种子上,接种后14 d测定相对根长、苗高、发芽率,并计算病情指数。结果表明,参试的15个菌株均显著降低了苜蓿种苗的根长(P<0.05)。总体来看,各菌株之间的致病性有所不同,从苜蓿品种WL-323HQ中分离出来的5号菌株的致病性最强,从WL-525HQ中分离出来的13号菌株的致病性最弱,从WL-525HQ、Legacy、Affinity和M illionaire中分离出来的12号、10号、8号和6号菌株的致病性较强,从Aohan、Farmers、WL-323M L和Dingdian中分离出来的1号、4号、7号和9号菌株的致病性较弱;参试的14个苜蓿品种之间的抗病性也各不相同,Amerimultileaf的抗病性最强,Amerileaf721、Victoria、Powerplant和Legacy的抗病性较强,Sandy、Phabulous、Hunter river和Alfaking的抗病性较弱,Vernal的抗病性最弱。
The objective of this study was to evaluate the pathogenicity of Fusarium acuminatum isolates to va- rious alfalfa varieties. Fourteen varieties of alfalfa were inoculated with 15 F. acuminamm isolates obtained from alfalfa roots collected at 4 locations. The relative root length, relative seedling height, relative germination rate and disease index were assayed at 14 days after inoculation. The results indicated that the root length of alfalfa seedlings was significantly reduced by all of the 15 isolates （P〈0.05）. Overall, the pathogenicity of each isolate varied among the 15 isolates. The isolate from ＇WL-323HQ＇ exhibited the strongest pathogenicity, while the isolate from ＇WL-525HQ＇ （planted in experimental area of Neihuang County of Anyang City） showed the weakest pathogenicity. The isolates from ＇WL-525HQ＇ （planted in experimental area in Zhengzhou City）, ＇Legacy＇ , ＇Affinity＇ and ＇Millionaire＇ showed strong pathogenicity, while the isolates from ＇Aohan＇, ＇ Farmers＇, ＇ WL-323ML＇ and ＇ Dingdian＇ showed weak pathogenicity. The disease resistance of the 14 alfalfa varieties also varied, among of them, ＇Amerimultileaf＇ showed the strongest resistance. ＇Amefileaf721 ＇, ＇ Victoria＇, ＇ Powerplant＇ and ＇ Legacy＇ exhibited high-level resistance. ＇ Sandy＇, ＇ Phabulous＇, ＇ Hunter fiver＇ and ＇Alfaking＇ showed low-level resistance. ＇Vernal＇ had the weakest resistance.
作者
郭玉霞
郭志鹏
张靖雪
王苗利
曲根
严学兵
张猛
GUO Yu-xia1, GUO Zhi-peng1, ZHANG Jing-xue1, WANG Miao-li1, QU Gen1, YAN Xue-bing1 , ZHANG Meng2(1 College of Animal and Veterinary Science, Henan Agricultural University, Zhengzhou 450002, China; 2College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Chin)
出处
《植物病理学报》
CAS
CSCD
北大核心
2018年第2期213-222,共10页
Acta Phytopathologica Sinica
基金
国家自然科学基金项目(31502006)
河南省高等学校重点科研项目(15A230019)
国家公益行业项目(201503112-1)
关键词
紫花苜蓿
锐顶镰孢
根腐病
致病性
抗病性
alfalfa
Fusarium acuminatum
root rot
pathogenicity
resistance
