摘要
目的:采用高效液相色谱法同时测定养心氏片中葛根素、大豆苷、丹参素、芦丁、延胡索乙素、淫羊藿苷、芹菜素、人参皂苷Rd、阿魏酸9个成分的含量。方法:采用Agilent Plus-C18色谱柱(250 mm×4.6 mm,5μm),检测波长:阿魏酸采用310 nm,其余8种成分采用254 nm,流动相为乙腈(A)和0.1%甲酸水(B),A相随时间的变化:5%~40%(0~35 min),40%(35~45 min);流速1 m L/min;柱温25℃;进样量5μL。结果:9个指标性成分葛根素、大豆苷、丹参素、芦丁、延胡索乙素、淫羊藿苷、芹菜素、人参皂苷Rd、阿魏酸,在35 min内实现完全分离,线性良好(r>0.9990)。方法学验证表明,日内日间精密度RSD<5%,加样回收率范围为98.91%~102.74%。结论:该方法简便、准确、实用性强,可用于养心氏片的质量控制。
Objective To determine the contents of nine marker components, including puerarin, daidzin, tanshinol, rutin, tetraby- dropalmatine, icariin, apigenin, ginsenoside -Rd, ferulic acid in Yangxinsihi Tablet by high performance liquid chromatography （HPLC） . Methods The HPLC condition was as follows ： column： Agilent Plus - C18 （250 mm × 4. 6 mm, 5 μm） ; The detective wave- length was set at 310nm for ferulic acid, and 254 nm for the other eight components. Mobile phase： A was ACN, B was 0. 1% formic acid aqueous solution, the gradient of A phase： 5% -40% （0 -35min）, 40% （35 -45min） ; flow rate： 1mL/min; temperature of column： 25℃; injection volume： 51xL. Results Nine marker components, including puerarin, daidzin, tanshinol, rutin, tetrabydropalma- tine, icariin, apigenin, ginsenoside - Rd, ferulic acid, were separated at baseline within 35 min with good linear （ r 〉 0. 9990） . The result of intra-day and inter- day precisions were at normal range （RSD 〈 5% ） . Range of the recovery was between 98.91% and 102. 74%. Conclusion The method is rapid, simple, accurate which can be used to control the quality of Yangxingshi Tablet.
作者
陈俊
徐圣弢
吕磊
吕昉
周燕妮
CHEN Jun XU Shengtao LV Lei LV Fang ZHOU Yanni(Department of Pharmacy, Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, Chin)
出处
《中国民族民间医药》
2018年第6期28-30,40,共4页
Chinese Journal of Ethnomedicine and Ethnopharmacy
