摘要
目的:探讨建立喉源性咳嗽模型的思路,找出更贴近临床的建模方法。方法:以多因素复合法建立喉源性咳嗽豚鼠模型A和B,比较两组模型的体质量、咳嗽次数、咽喉及肺组织病理组织、血液指标、特异性炎性因子等结果,总结出更贴近临床特点的建模方法。结果:与正常组比较,两模型组体质量均有不同程度的下降,模型B组差异明显(P<0.05);咳嗽次数均有显著增加(P<0.01);咽喉、肺组织病理切片均显示存在不同程度的炎性反应,模型B组炎性反应较重;血嗜酸性细胞比例有不同程度的上升,模型A组差异明显(P<0.05);T细胞免疫相关的特异性炎性因子出现异常,模型A组差异明显(P<0.05,P<0.01)。结论:在氨水+松节油喷雾法的基础模型上,复合烟熏+局部滴菌+枸橼酸诱发法建立喉源性咳嗽模型,是更贴近临床特点的造模方法。
Objective： To discuss the attitudes of establishing the model of laryngeal cough and to provide a more appropriate modeling method for clinical practice. Methods： Guinea pig model A and B of laryngeal cough were established by multifactor of simulated causes. With a series of tests, such as weight, cough numbers, pathological observations of pharynx, larynx and lung tissues, blood indexes and specific inflammatory factors, a more appropriate modeling method for clinical practice was concluded. Results： Compared with the normal group, the body weight of the two groups decreased in different levels, while model group B had statistic difference（P〈0.05）; the cough numbers increased in both model groups in different levels（P〈0.01）; inflammatory reaction existed in both model groups in different levels from the tissue samples of pharynx, larynx and lung, and model group B had more severe inflammatory reaction; eosinophilic granulocyte ratio of the blood increased in both model groups in different levels, while model group A had statistic difference（P〈0.05）; specific inflammatory factors related to T cell immunity in both model groups were different, and model group A had a more significant difference（P〈0.05, P〈0.01）. Conclusion： In the basic model of ammonia plus turpentine spray method, laryngeal cough model can be established by compound smoking, localized bacteria dropping and citric acid, which is proved to be more pragmatic and appropriate for clinical practice.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第3期903-906,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81473596)
浙江省自然科学基金项目(No.Q15H270014)~~
