摘要
目的:研究山核桃叶总黄酮对小鼠胚胎间充质干细胞C3H10T_(1/2)成脂分化的影响。方法:建立C3H10T_(1/2)细胞成脂分化模型,油红O染色法分析山核桃叶总黄酮对C3H10T_(1/2)细胞成脂分化的影响;GAP-PAP酶法测定C3H10T_(1/2)细胞成脂分化过程中甘油三酯含量变化;采用荧光定量PCR和Western Blot检测过氧化物酶体增殖物激活受体γ(PPARγ)、CCAAT增强子结合蛋白α(C/EBPα)以及脂肪型脂肪酸结合蛋白4(FABP4)的mRNA和蛋白表达水平。结果:山核桃叶总黄酮能抑制C3H10T_(1/2)细胞成脂分化,20μg/m L山核桃叶总黄酮能显著减少甘油三酯的堆积,降低PPARγ、C/EBPα、FABP4的mRNA和蛋白表达水平。结论:山核桃叶总黄酮对C3H10T_(1/2)细胞成脂分化具有抑制作用,其机制可能与抑制PPARγ、C/EBPα、FABP4的表达有关。
Objective： To study the effect of total flavonoids from Carya cathayensis leaves on adipogenic differentiation of mice embryonic mesenchymal stem cell C3H10T_（1/2）.Methods： The model of adipogenic differentiation of C3H10T_（1/2） was established,the effect of total flavonoids from Carya cathayensis leaves on adipogenic differentiation of C3H10T_（1/2） was determined by oil red O staining.GAPPAP enzymatic was used to detect the content of triglyceride.Real-time PCR and Western blot were employed to observe the mRNA and protein expression levels of PPARγ,C/EBPα,FABP4,respectively.Results： Total flavonoids from Carya cathayensis leaves could inhibited the adipogenic differentiation of C3H10T_（1/2）,and in dose of 20 μg/m L can significantly reduce the the level of triglyceride,decreased the mRNA and protein expressions of PPARγ,C/EBPα,FABP4.Conclusion： The results indicate that total flavonoids from Carya cathayensis leaves has a suppressive effect on adipogenic differentiation of C3H10T_（1/2）,the mechanism may be associated with the inhibition of the expression of PPARγ,C/EBPα and FABP4.
出处
《中药材》
CAS
北大核心
2017年第5期1174-1177,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81303258)
浙江省科技计划资助项目(2017C33107)
关键词
山核桃叶总黄酮
小鼠胚胎间充质干细胞
成脂分化
Total flavonoids from Carya cathayensis leaves
Mice embryonic mesenchymal stem cell
Adipogenic differentiation
