摘要
依据菲律宾蛤仔血细胞的外部形态、凝集程度,比较了10种抗凝剂的抗凝效果,并研究了抗凝效果好的抗凝剂对血细胞存活率、血细胞组成、血细胞凋亡率的影响。试验结果显示,抗凝剂1、抗凝剂9与抗凝剂10对菲律宾蛤仔血细胞的抗凝效果较好,6h内3种抗凝剂中的血细胞存活率均在94.5%以上,且各组之间无显著性差异;3种抗凝剂中的血细胞2h时颗粒细胞占比与固定液组相比无显著性差异,6h时抗凝剂1中颗粒细胞占比显著升高,而抗凝剂9和抗凝剂10中的颗粒细胞占比显著下降;而对于血细胞凋亡率,2h时抗凝剂10中的血细胞的凋亡率显著上升,其余两组无明显变化,而6h时除抗凝剂1中细胞凋亡率与对照组无显著差异外,抗凝剂9和抗凝剂10中血细胞凋亡率均显著上升。利用透射电镜和扫描电镜观察抗凝剂1中6h时血细胞的形态结构,结果发现其表面和内部超微结构保存亦较好。试验结果表明,抗凝剂1较适合作为菲律宾蛤仔血细胞的抗凝剂。
The anticoagulant effects of 10 kinds of anticoagulants were compared in Manila clamRuditapes philippinarumbased on haemocyte shape and the agglutination degree,and the effects of the anticoagulants with good anticoagulant effect on the haemocyte survival rate,composition and the apoptosis rate were studied.The results showed that the anticoagulant 1,anticoagulant 9 and anticoagulant 10 had good anticoagulant effects.The haemocyte survival rates were of more than 94.5%in the three anticoagulant groups without significant difference between these groups（P〈0.05）.There was no significant change in proportion of granulocytes in the three anticoagulant groups within 2 h（P〈0.05）,signifcant increase in anticoagulant 1 group,and significant decrease in both anticoagulant 9 and anticoagulant 10 in 6 h（P〈0.05）.as to haemocyte apoptosis rate.There was a signifcant increase in haemocyte apoptosis in anticoagulant 10 in 2 h（P〈0.05）,and without significant change in other two groups（P〉0.05）.In 6 h,except for the anticoagulant 1,the haemocyte apoptosis rate was significantly increased in anticoagulant 9 and anticoagulant 10 groups（P〈0.05）.The scanning electron microscopy and transmission electron microscopy revealed that the haemocyte external and internal ultrastructures were better preserved in anticoagulant 1 in 6 h.The findings indicated that anticoagulant 1 was a desirable anticoagulant for haemocytes.
出处
《水产科学》
CAS
CSCD
北大核心
2018年第1期24-30,共7页
Fisheries Science
基金
国家自然科学基金资助项目(31302166)
浙江省近岸水域生物资源开发与保护重点实验室开放基金资助项目(J2013004)
关键词
菲律宾蛤仔
血细胞
抗凝剂
筛选
Ruditapes philippinarum
haemocyte
anticoagulant ~ screen
