摘要
通过对固相微萃取条件、气相色谱/质谱条件的优化,建立了顶空-固相微萃取-气质联用(HS-SPME-GC/MS)方法分析酸梅膏中挥发性香气成分,并采用该方法分析了6种酸梅膏中的挥发性香气成分。0.50 g酸梅膏样品,用粉色萃取头在40℃下萃取20.0 min,解吸3.0 min后顶空进样,采用弹性石英毛细管柱在全扫描监测模式下进行GC/MS分析。结果表明,6种酸梅膏共检测到36种挥发性香气成分,包括酯类9种、酮类9种、酸类5种、醇类5种、醛类3种、酚类3种、杂环1种、烃类1种;主要挥发性香气成分为β-紫罗兰酮和二氢-β-紫罗兰酮;β-紫罗兰酮、丙位癸内酯、茶香螺烷、山梨酸乙酯、山梨酸和糠醛为共检出挥发性香气成分。该方法前处理简单、结果准确可靠,适用于酸梅膏中挥发性香气成分的分析检测。
Through optimizing the conditions of solid phase microextraction and gas chromatography-mass spectrometry, we established headspace-solid phase microextraction-gas chromatography-mass spectrometry （HS-SPME-GC/MS） method to analyse the volatile aroma components in sour plum juice,and analyzed the volatile aroma components in six sour plum juices by this method. 0.50 g sour plum juice was extracted with the pink extraction head for 20.0 min at 40 ℃, and injected by headspace sampling after desorbing for 3.0 min. Then,GC/MS analysis was applied with fused silica capillary column in full scan mode. The results showed that 36 compounds were identified in 6 samples,including 9 esters,9 ketones,5 acids,5 alcohols,3 aldehydes,3 phenols,1 heterocyclic, and 1 hydrocarbon. The main volatile aroma components were β-ionone and dihydro-β-ionone. β-ionone, gamma-decalactone, theaspirane, sorbic acid ethyl ester, sorbic acid, and furfural were all detected in 6 samples. This method is simple, accurate, reliable, and suitable for the analysis of volatile aroma components in sour plum juice.
出处
《化学与生物工程》
CAS
2018年第1期62-67,共6页
Chemistry & Bioengineering
基金
云南省科技厅青年项目(2014FD079
2015FD098)
云南中烟工业有限责任公司技术中心科技项目(JSZX2014JC05)
关键词
酸梅膏
挥发性香气成分
顶空
固相微萃取
气质联用法
sour plum juice
volatile aroma component
headspace
solid phase microextraction
gas chromatography-mass spectrometry
