摘要
目的观察电针"曲池"、"足三里"对脑缺血模型大鼠缺血皮层中突触素(SYN)及脑源性神经营养因子(BDNF)表达的影响,探讨电针治疗缺血性脑卒中的作用机制。方法 90只大鼠随机分为假手术组、模型组、电针组,按时间点每组又分为3个亚组:3天组、7天组、14天组,每亚组10只大鼠。模型组和电针组均用改良的线栓法建立大鼠左侧大脑中动脉阻塞模型。各组于术后2 h及处死前行神经功能缺损评分。电针组予电针患侧"曲池"穴和"足三里"穴,1次/天,每次30 min,至动物处死。采用免疫组织化学染色、Western blot和逆转录-聚合酶链反应(RT-PCR)检测大鼠左侧皮质SYN、BDNF表达情况。结果与假手术组比较,模型组、电针组神经功能缺损评分明显升高(P<0.01);经电针治疗后,电针组神经功能缺损评分低于模型组(P<0.05)。免疫组织化学染色和Western blot结果显示,与假手术组比较,模型组、电针组SYN蛋白表达显著降低,电针组SYN蛋白表达比模型组显著提升(P<0.01);与假手术组比较,模型组、电针组BDNF蛋白表达显著上升,电针组BDNF蛋白表达比模型组显著增多(P<0.01)。RT-PCR结果表明,与假手术组比较,模型组、电针组SYN mRNA表达显著下降,电针组SYN mRNA表达比模型组显著升高(P<0.01);与假手术组比较,模型组、电针组BDNF mRNA表达显著升高,电针组BDNF mRNA表达比模型组显著升高(P<0.01)。结论电针"曲池"、"足三里"可促进脑缺血大鼠皮质BDNF的合成和分泌,上调SYN的表达,可能在调控脑可塑性方面发挥重要作用。
Aim To observe the effect of electroacupuncture（EA） at Quchi and Zusanli acupoint on the expressions of cortical synaptophysin（SYN） and brain-derived neurotrophic factor（BDNF） in cerebral ischemia model rats,and to explore the mechanism of EA in the treatment of ischemic stroke. Methods 90 rats were randomly divided into sham operation group,model group and EA group,and each group was divided into 3 subgroups according to time points： 3 days group,7 days group,14 days group,10 rats in each subgroup. Left middle cerebral artery occlusion model was established by modified suture plug method in model group and EA group. Neurological deficit score was scored at 2 h after operation and before death. EA group received EA at affected side Quchi and Zusanli acupoint,1 time each day,30 minutes each time,until the animal was killed. Immunohistochemical staining,Western blot and reverse transcription polymerase chain reaction（RT-PCR） were used to detect the expression of SYN and BDNF in the left cortex of rats. Results Compared with sham operation group,the neurological deficit scores of model group and EA group were significantly increased（P〈0.01）; after EA treatment,the neurological deficit score of EA group was lower than that of model group（P〈0.05）. Immunohistochemical staining and Western blot results showed that compared with sham operation group,the expression of SYN protein was significantly decreased in model group and EA group,and the expression of SYN protein in EA group was significantly higher than that in model group（P〈0.01）; Compared with sham operation group,the expression of BDNF protein was significantly increased in model group and EA group,and the expression of BDNF protein in EA group was significantly higher than that in model group（P〈0.01）. RT-PCR results showed that compared with sham operation group,the expression of SYN mRNA was significantly decreased in model group and EA group,and the expression of SYN mRNA in EA group was significantly higher than that in model group（P〈0. 01）; Compared with sham operation group,the expression of BDNF mRNA was significantly increased in model group and EA group,and the expression of BDNF mRNA in EA group was significantly higher than that in model group（P〈0.01）. Conclusion Electroacupuncture at Quchi and Zusanli acupoint can promote the synthesis and secretion of BDNF in the cerebral cortex of rats with cerebral ischemia,and increase the expression of SYN,which may play an important role in the regulation of brain plasticity.
出处
《中国动脉硬化杂志》
CAS
北大核心
2017年第11期1099-1106,共8页
Chinese Journal of Arteriosclerosis
基金
福建省自然科学基金(2017J01829)
福建省医学创新课题(2016-CX-47)
福州市科技局课题(2016-G-49)
关键词
电针
“曲池”穴
“足三里”穴
脑缺血
突触素
脑源性神经营养因子
Electroacupuncture
Quchi acupoint
Zusanli acupoint
Cerebral ischemia
Synaptophysin
Brain-derived neurotrophic factor
