摘要
目的:提取分离骆驼刺茎枝粗多糖,测定其理化性质及抗氧化活性。方法:采用水提醇沉法提取骆驼刺茎枝粗多糖,硫酸苯酚法测定总糖含量,考马斯亮蓝法测定蛋白含量,咔唑硫酸法测定糖醛酸含量,GC法测定单糖组成及相对摩尔比;通过测定骆驼刺茎枝多糖的还原力,及其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基和羟自由基的清除力,评价骆驼刺茎枝多糖的体外抗氧化活性。结果:水提醇沉制备的骆驼刺茎枝粗多糖总糖含量为73.2%,其中糖醛酸占粗多糖总糖含量的27.2%;蛋白质含量为17.6%;骆驼刺茎枝多糖由Rha、Ara、Xyl、Man、Glc、Gal、Glc A和Gal A 8种单糖组成,相对摩尔比为1.05∶1.00∶1.25∶0.52∶3.05∶1.31∶0.47∶4.78;骆驼刺茎枝多糖的还原力、对DPPH与羟基自由基的清除率随多糖浓度的增大而增强。结论:初步提取分离了骆驼刺茎枝粗多糖,研究了其理化性质和体外抗氧化活性,为骆驼刺的开发利用提供研究基础。
Objective： To study the extraction, separation, physical and chemical properties and antioxidant activity of the crude polysaccharide from Alhagi sparsifolia Shap. stem-branch. Methods： The crude polysaccharide from Alhagi sparsifolia Shap. stem- branch was extracted by ethanol subsiding method. The total sugar content was determined by phenol-sulfuric acid method and the pro- tein content was determined by coomassie brilliant blue method. The content of uronic acid was determined by carbazole sulfuric acid method and the monosaccharide composition and the relative molar ratio were determined by GC. The antioxidant activities in vitro were evaluated by determining the reducing power of polysaccharide from Alhagi sparsifolia Shap stem-branch and its removal ability to 1,1- diphenyl-2-trinitrophenylhydrazine （DPPH） radicals and hydroxyl radicals. Results： The total sugar content of crude polysaccharide from Alhagi sparsifolia Shap. stem-branch was 73.2%, and uronic acid accounted for 27.2% of the total sugar content of crude poly- saccharide. The content of protein was 17.6%. The crude polysaccharide from Alhag4 sparsifolia Shap. stem-branch was composed of Rha, Ara, Xyl, Man, Glc, Gal, GlcA and GalA, and the relative molar ratio was 1.05： 1.00：1.25： 0.52：3.05： 1.31 ： 0.47： 4.78. The reducing power of the polysaccharide from Alhagi sparsifolia shap. stem-branch and the clearance rate on DPPH radicals and hy- droxyl radicals increased with the increase of polysaccharide concentration. Conclusion： The crude polysaecharide from Alhagi sparsi- folia Shap. stem-branch was extracted, and the physica! and chemical properties and antioxidant activity in vitro were studied, which provide foundation for the further investigation and comprehensive utilization of Alhagis parsifolia Shap. stem-branch.
出处
《中国药师》
CAS
2018年第1期48-52,共5页
China Pharmacist
基金
陕西省科技统筹创新工程计划项目(编号:2016KTCL03-14)
关键词
骆驼刺茎枝粗多糖
单糖组成
理化性质
抗氧化活性
Crude polysaccharide from Alhagi sparsifolia Shap. stem-branch
Monosaccharide composition
Physical and chemi- cal properties
Antioxidant activity
