摘要
目的探讨不同缺氧时间点喉癌Hep-2细胞缺氧诱导因子-1α（HIF-1α）、葡萄糖转运蛋白1（GLUT-1）、基质金属蛋白酶-2（MMP-2）的表达。方法MTT法检测不同缺氧时间点细胞增殖状况，Transwell法检测缺氧对喉癌Hep-2细胞侵袭能力的影响，RT-PCR和Western blot检测缺氧后不同时间点HIF-1α、GLUT-1、MMP-2的mRNA及蛋白的表达情况。结果随着缺氧时间的延长，光密度值逐渐升高；缺氧培养24 h、48 h时细胞侵袭力较正常条件下的侵袭力明显升高（P〈0.05）；缺氧0 h时，细胞即有HIF-1α、MMP-2、GLUT-1的mRNA表达，随着缺氧时间延长，上述指标除HIF-1α外，其余指标mRNA均呈明显增强趋势（P〈0.05），Western blot法结果显示，在正常条件下，即有HIF-1α、MMP-2、GLUT-1mRNA的表达，随着缺氧时间延长，上述指标水平均升高。结论缺氧环境能增强喉癌Hep-2细胞活性和侵袭能力，缺氧可上调HIF-1α、GLUT-1、MMP-2的表达，喉癌细胞在缺氧下可通过三者的表达以满足自身生长、增殖的需求，促进喉癌的发展。
Objective To investigate the expression of hypoxia inducible factor-1α（HIF-1α）, glucose transporter 1（GLUT-1） and matrix metalloproteinase-2 （MMP-2） in laryngeal carcinoma Hep-2 cells at different time points.MethodsMTT assay was used to detect the cell proliferation at different time points of hypoxia, the effects of hypoxia on invasive ability of laryngeal carcinoma Hep-2 cells was detected by transwell, the expression of mRNA of HIF-1α, GLUT-1 and MMP-2 and protein were detected by RT-PCR and Western blot method.ResultsWith the duration of hypoxia, the optical density value increased gradually; the cell invasive ability with hypoxia for 24 h, 48 h was higher than invasive ability under normal conditions （P〈0.05）; when hypoxia for 0 h, mRNA expression of HIF-1α and MMP-2, GLUT-1 existed, with the duration of hypoxia, the mRNA of above indexes except HIF-1α significantly increased （P〈0.05）, Western blot assay showed that under normal conditions, the mRNA expression of HIF-1α, MMP-2 and GLUT-1 had existed, with the duration of hypoxia, the above indexes significantly increased.ConclusionsHypoxia can enhance the activity and invasion of laryngeal carcino-ma Hep-2 cells, and up-regulate the expression of HIF-1α, GLUT-1, MMP-2; laryngeal carcinoma Hep-2 cells can meet the need of growth and proliferation by the expression of the three genes in hypoxia so as to promote the development of laryngeal carcinoma.
出处
《中国实用医刊》
2017年第22期74-76,共3页
Chinese Journal of Practical Medicine
关键词
喉癌
HEP-2细胞
缺氧
不同时间点
上调
Laryngeal carcinoma
Hep-2 cells
Hypoxia
Different time points
Up-regulation
