摘要
目的探讨EGFR在鼻咽癌临床分期中的作用。方法将鼻咽未分化非角化癌60例及鼻咽良性病变(慢性鼻咽炎及腺样体组织)30例的病理组织,利用免疫组化二步法检测其中EGFR的表达情况,结果经IPP6.0进行图像分析,并将检测结果与临床病理特征进行综合分析。结果 EGFR在鼻咽癌及慢性鼻咽炎病理组织中,都存在EGFR的表达,并在鼻咽未分化非角化癌组织中高表达,其MOD(EGFR)与鼻咽良性病变组织相比,存在显著性差异(p=0.004<0.01),并且MOD(EGFR)与鼻咽癌的临床分期存在正相关性,相关系数0.786,鼻咽癌组织EGFR的表达与T分期正相关,相关系数0.722,与N分期存在一定相关性,N2与N3之间MOD(EGFR)无显著性差异(p>0.05),剩余组间比较均有统计学意义(p<0.05),并且MOD(EGFR)随着N分期的升高,有逐渐升高的趋势,但N分期与MOD(EGFR)的相关系数仅为0.250;结论在鼻咽癌及鼻咽良性病变病理组织中,都存在EGFR的表达,但在鼻咽癌组织中高表达,EGFR表达可能与鼻咽癌肿瘤细胞增殖活跃度密切相关;鼻咽病理组织中EGFR的免疫组化测定作为一种判断鼻咽肿瘤细胞增殖情况的指标。
Objective To investigate the role of EGFR in the clinical grading of nasopharyngeal carcinoma（NPC）.Methods Examining the expression of EGFR by Two-step Method in immunohistochemistry on the pathological tissue specimens from 60 NPC patients and 30 patients with benign nasopharyngeal diseases, the result was analyzed by the IPP6.0. The clinical pathological features of these specimens were also taken into account.Results The expression of EGFR was found in the pathological tissue specimens of both NPC patients and chronic nasopharyngitis patients but higher in the specimens of NPC patients. The MOD（EGFR） of NPC specimens have significant difference（p=0.0040.01） with chronic nasopharyngitis specimens. Also, MOD（EGFR） has positive correlation（correlation coefficient=0.786） with clinical staging of nasopharyngeal carcinoma.The expression of EGFR in nasopharyngeal carcinoma tissue was positively correlated with T scale（correlation coefficient=0.722）. It was also correlated with N scale, there were no significant difference（p0.05） between MOD（EGFR） of N2 and N3, but significant difference（p0.05） was found between other groups, the MOD（EGFR） gradually increased with the upgrade of N scale, but the correlation coefficient between N scale and MOD（EGFR） was only 0.250; Conclusion The expression of EGFR was found in the pathological tissue specimens of both NPC patients and chronic nasopharyngitis patients but higher in the specimens of NPC patients. The expression of EGFR may be closely related to the proliferation vitality of NPC tumor cells. As an indicator of nasopharyngeal tumor cell proliferation, immunohistochemical exanmination of EGFR worth popularizing in clinical practice.
出处
《佛山科学技术学院学报（自然科学版）》
CAS
2017年第6期62-67,共6页
Journal of Foshan University(Natural Science Edition)
基金
广东省佛山市卫生局医学科学技术研究基金(2015218)
关键词
鼻咽癌
表皮生长因子受体
免疫组织化学
显微图像分析
nasopharyngeal carcinoma
epidermal growth factor receptor
immunohistochemistry
microscopicimage analysis
