摘要
目的 观察党参提取物 (D h)和白术提取物 (B t)对小肠隐窝细胞 (IEC 6 )增殖、分化的作用及其二者配伍对IEC 6细胞增殖的影响。方法 IEC 6细胞培养 2 4h ,加入不同剂量的D h、B t及二者的配伍溶液 ,2 4h后用MTT法观察细胞增殖 ,显微镜观察细胞分化的形态特征。结果 D h在 2 5 0mg·L-1以下剂量时对细胞增殖无影响 ,剂量提高到 5 0 0mg·L-1和 10 0 0mg·L-1时 ,明显促进细胞的增殖。B t各剂量均无促进细胞增殖的作用 ,在 5 0 0mg·L-1和10 0 0mg·L-1剂量时 ,细胞增殖反见减弱。二药配伍后 ,促进细胞增殖的作用明显增强 ,具有一定的剂量依赖关系 ,其效果优于D h ;IEC 6细胞在B t作用下 ,分化程度增高 ,低倍镜下细胞呈典型的上皮细胞形态 ,细胞排列呈条索状 ,部分呈腺管状排列。高倍镜下可观察到隐约有细胞连接样结构 ,上皮细胞似有微绒毛形成的趋势。D h处理的细胞分化程度较低 ,镜下所见多为未分化的梭状细胞 ,且排列不规则 ,无任何形成腺管的趋势和有细胞连接样结构。结论 D h能促进细胞增殖 ,B t可促进细胞分化 ,二者配伍后促进细胞增殖作用明显增强 。
AIM To investigate the effects of extracts from dangshen (Codonopsis pilosula,D h) and baizhu (Atractylodes macrocephala, B t) individually and in combination on the proliferation and differentiation of rat small intestinal crypt like cell line (IEC 6). METHODS IEC 6 cells were plated and treated with D h, B t or D h+B t at 24 h to 48 h. The cell proliferation was estimated by MTT assay and the cell differentiation was observed under microscope after HE stainning. RESULTS Compared with control,D h promoted the cell growth in a dose dependence manner. While B t induced the cell differentiation. Treatment with D h plus B t resulted in a more significant increase of the cell proliferation than treatment with D h alone. CONCLUSION D h and B t both have effect on IEC 6 cells in which D h stimulated proliferation yet B t promoted differentiation. A treatment of D h plus B t stimulated IEC 6 cell proliferation more evidently than D h treatment alone suggesting that these two extracts have a synergistic action on IEC 6 cell proliferation.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2002年第4期444-447,共4页
Chinese Pharmacological Bulletin
基金
国家重点基础研究发展规划资助项目 (国家 973计划 :NoG19990 5 44 )
国家自然科学基金项目 (No 3 9970 90 6)。
