摘要
目的探究人参白虎汤对1型糖尿病(T1DM)幼鼠血糖及干扰素γ(IFN-γ)、白细胞介素4(IL-4)的影响作用。方法将50只幼鼠随机选取10只设为正常组,剩余40只以链脲佐菌素(STZ)腹腔注射1次诱导T1DM模型,造模成功后随机分为模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组,每组各10只。胰岛素组予精蛋白生物合成人胰岛素注射液2 U/(kg·d)皮下注射,人参白虎汤高、低剂量组分别予人参白虎汤12、6 mL/(kg·d)灌胃,正常组及模型组予0.9%氯化钠注射液1 mL灌胃,均持续6周。观察5组幼鼠给药前后体质量变化,分别于给药前及给药结束后采集5组幼鼠尾静脉血测定空腹血糖,处死幼鼠取腹主动脉血采用酶联免疫吸附(ELISA)法检测IFN-γ和IL-4水平,并观察5组幼鼠胰腺的病理变化。结果给药前,模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组幼鼠体质量均较正常组明显下降(P<0.05),空腹血糖升高(P<0.05);给药后,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组幼鼠体质量均较本组给药前及模型组同期升高(P<0.05),空腹血糖降低(P<0.05),但与正常组同期比较差异仍有统计学意义(P<0.05);与胰岛素组相比,人参白虎汤高剂量组体质量及空腹血糖无明显差异(P>0.05),而人参白虎汤低剂量组体质量低,空腹血糖高(P<0.05)。与正常组相比,模型组、胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组IFN-γ水平升高,IL-4水平降低(P<0.05);与模型组相比,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组IFN-γ水平明显降低(P<0.05),IL-4水平明显升高(P<0.05),且人参白虎汤高剂量组与胰岛素组相比比较差异无统计学意义(P>0.05)。胰腺组织病理观察显示,胰岛素组、人参白虎汤高剂量组及人参白虎汤低剂量组胰岛β细胞损伤较模型组减轻,但仍未恢复正常。结论人参白虎汤能明显降低T1DM幼鼠血糖、IFN-γ水平,提高IL-4水平,改善胰腺病理结构,进而发挥修复T1DM幼鼠胰岛免疫损伤的作用。
Objective To observe the effects of Renshen-baihu decoction on plasma glucose,IFN – γ and interleukin-4( IL-4) of immature rats with type 1 diabetes mellitus( T1 DM). Methods 10 rats were randomly selected from 50 rats as the normal group,the remaining 40 rats were injected intraperitoneally with streptozotocin( STZ) to induce T1 DM model. After successful modeling,the rats were randomly divided into model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group,and 10 rats in each group. The insulin group was was injected subcutaneously with insulin by 2 U/( kg·d). The high and low dose of Renshen-baihu decoction groups were respectively given Renshen-baihu decoction gavage by 12 mL/( kg·d) and 6 mL/( kg·d). The normal group and model group were given 1 mL 0. 9% sodium chloride injection gavage. The treatment course was continuously for 6 weeks. The changes of body weight of immature rats before and after administration were observed in 5 groups. The tail vein blood was collected to detect fasting blood glucose before and after the administration in 5 groups. The abdominal aorta blood was collected to detect he levels of IFN-γ and IL-4 by enzyme-linked immunosorbent assay( ELISA) and the pathological changes of pancreas of immature rats were observed. Results The body weight before administration in model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were significantly lower than those in normal group( P < 0. 05),and fasting blood glucose increased( P < 0. 05). The body weight after administration in the insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were higher than those before administration and those in the model group at the same period( P < 0. 05),and the blood glucose was decreased( P < 0. 05),and had statistical differences with the normal group at the same period( P < 0.05). Compared with the insulin group,there was no significant difference on the body weight and fasting blood glucose in the high dose of Renshen-baihu decoction group( P > 0. 05),while the the body weight in the low-dose of Renshen-baihu decoction group was lower and the fasting blood glucose was still higher( P < 0. 05). Compared with the normal group,the IFN-γ increased in model group,insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group,and IL-4 reduced( P < 0. 05). Compared with the model group,the IFN-γ in insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were significantly decreased( P < 0. 05),IL-4 was significantly increased( P < 0. 05),and there was no significant difference between high dose of Renshen-baihu decoction group and insulin group( P > 0. 05).The observation of pancreatic tissue histopathology showed that the injury of islet β cell in the insulin group,high dose of Renshen-baihu decoction group and low dose of Renshen-baihu decoction group were less than that in model group,but still not returned to normal. Conclusion Renshen-baihu decoction can significantly reduce the blood glucose,IFN-γ,and increase IL-4 in immature rats with T1DM,improve the pathological structure of the pancreas,and thus can play a role in the repair of islet immune injury in immature rats.
出处
《河北中医》
2017年第11期1700-1705,共6页
Hebei Journal of Traditional Chinese Medicine
