摘要
为了探讨适宜高压电场处理高粱种子促进种子萌发的机制,以晋杂122号高粱种子为试材,采用完全随机试验设计方法,研究高压电场处理对种子萌发期α-淀粉酶、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)、过氧化物酶(POD)活性的影响。结果表明,高粱种子经适宜高压电场处理后,萌发活力均有提高,其中,T4处理(500 k V/m×30 min)提高幅度最大,T5处理(500 k V/m×40 min)次之;各处理高粱种子萌发期的α-淀粉酶活性均高于对照(未经电场处理),且T4处理最高,为3.80 mg/(g·min),比对照提高了156.75%;各处理高粱种子萌发期的CAT活性与对照相比变化不一致,仅T2(400 k V/m×40 min)、T5、T8(600 k V/m×40 min)3个处理高于对照;除T8处理高粱种子萌发期的POD活性与对照接近外,其余各处理均高于对照,以T3处理(400 k V/m×50 min)最高,为115.81 U/(g·min),比对照提高了69.16%;各处理高粱种子萌发期的SOD活性均高于对照,其中T4处理最高,为774.45 U/g,比对照提高了175.31%,T2、T5处理次之,分别比对照提高了171.93%、100.05%。可见,适宜电场处理高粱种子可提高种子内α-淀粉酶、SOD、POD活性,促进种子萌发。
In order to explore the reason why appropriate high voltage electric field（HVEF） can promote sorghum seed germination, the experiment was designed to decipher the possible mechanism. This experiment used the Jinza 122 as test material and the completely random design of experiment as method, and mainly focused on the change of α-amylase, CAT, SOD, POD activity of sorghum seeds. The results showed that the seed vigor of sorghum seeds increased with the treatment of HVEF. Further study showed that when the electrostatic field was 500 kV/m and the treatment time was 30min （ T4 ） , the germination comprehensive index value reached maximum, followed by T5 （500 kV/m × 40min）. Compared to the control group （not treated by HVEF） , all test groups showed higher α-amylase activity and among them T4 showed the highest α-amylase activity,which was 3.80 mg/（g ·min） and increased by 156.75% compared to the control group. The CAT activity of all the test groups decreased except T2 （400 kV/m ×40 min） ,Ts,Ts （600 kV/m × 40 min）. All POD activities of test groups were higher than control group except Ts, which was similar to the control group. T3 showed the highest POD activity, which was 115.81 U/（g · min） and increased by 69. 16% compared to the control group. The SOD activities of all the test groups were higher than that of control, and T4 reached maximum value, which was 774.45 U/g and increased by 175.31% ,followed by T2 and T5 ,which were increased by 171.93% and 100.05%. The results indicate that appropriate HVEF can promote sorghum seed germination by increasing the α-amylase activity and protective enzyme activity.
出处
《河南农业科学》
CSCD
北大核心
2017年第11期30-34,共5页
Journal of Henan Agricultural Sciences
基金
山西省农业科技攻关项目(20130311014-3)
