摘要
目的:建立UPLC-MS/MS同时测定苦苣苔科植物中7个苯乙醇苷类成分的方法,分析比较桂林产半蒴苣苔属植物中苯乙醇苷含量差异。方法:采用ACQUITY UPLC BEH C18色谱柱(100 mm×2.1 mm,1.8μm),以0.1%甲酸溶液(A)-0.1%甲酸乙腈溶液(B)为流动相,梯度洗脱,流速为0.6 m L/min;柱温40℃;采用电喷雾离子源,负离子检测方式,得到相应的提取离子流图,以峰面积进行定量。结果:7个苯乙醇苷类成分具有良好的线性关系,r值均大于0.9990,方法精密度、重复性和稳定性的RSD值均小于3.5%,加样回收率在97.1%~103.2%,RSD均小于3.6%。14种半蒴苣苔属植物中7个苯乙醇苷在组成和含量上有一定差异。结论:该方法操作简便、准确、重复性好,对寻找高含量苯乙醇苷类资源植物具有指导意义。
Objective：To establish an UPLC-MS/MS method for the simultaneous determination of seven phenylethanol glycosides in Gesneriaceae species, and to analyze the difference of phenylethanol glycosides ingredients in the samples from Guilin habitats. Methods：The determination was performed on the AQUITY BEH Cls （100 mm × 2. 1 mm, 1.8 μm） ;the mobile phase was 0. 1% formic acid （A） -0. 1% formic acid acetonitrile （B） in gradient elution; the flow rate was 0. 6 mL/min; the column temperature was 40 ℃. MS instrument was equipped with ESI-ion source. Gaining the extracted ion chromatograms, then the peak area was used for quantitative. Results： Seven phenylethanol glycosides showed good linearity（ r ≥0. 9990）. The RSD of the precision, repeatability, and stability tests were less than 3.5%. The average recovery rates were in the range of 97. 1% -103. 2% ,the RSD were less than 3.6%. There are differences in the composition and content of seven phenylethanol glycosides from different Gesneriaceae species. Conclusion ：The method for the sim- ultaneous determination of the seven phenylethanol glycosides is simple, accurate, and reproducible,which has great theoretical signifi- cance for looking for high levels of phenylethanol glycosides of plant resources.
出处
《中药材》
CAS
北大核心
2017年第4期869-872,共4页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金(81373959)
中医药行业科研专项(201407003)
2016年北京中医药大学自主选题(在读研究生项目)(2016-JYB-XS125)
