摘要
目的 观察阿帕替尼对食管癌Kyse-150细胞放射敏感性的影响，并初步探讨其作用机制。方法 将食管癌Kyse-150细胞分为空白对照组、阿帕替尼组、单纯照射组、阿帕替尼联合照射组。CCK-8法检测不同浓度阿帕替尼（0、5、10、20、30、40 μmol/L）对细胞增殖的影响；克隆形成法检测不同浓度阿帕替尼（0、10、20、40 μmol/L）对细胞放射敏感性的影响；流式细胞术分析阿帕替尼（20 μmol/L）联合射线（4 Gy）对细胞周期和细胞凋亡的影响。结果 阿帕替尼能抑制Kyse-150细胞的增殖，且呈剂量-时间依赖性（r=0.89~0.96，P〈0.05）；随着阿帕替尼浓度的增加，Kyse-150细胞的放射生物学参数D0、Dq、SF2值逐渐减小，而放射增敏比SERD0值逐渐增加。阿帕替尼联合照射组分别与空白对照组、单纯照射组及阿帕替尼组比较，细胞凋亡率均显著增加，差异具有统计学意义（t=12.36、5.99、15.47，P〈0.05）。与空白对照组比较，阿帕替尼组、单纯照射组及阿帕替尼联合照射组G2/M期比例均增高，差异均具有统计学意义（t=8.81、39.69、20.61，P〈0.05）。阿帕替尼组、阿帕替尼联合照射组分别与空白对照组及单纯照射组相比，S期比例均增高，差异均具有统计学意义（t=6.06、3.82、8.81、6.24，P〈0.05）。而阿帕替尼组与阿帕替尼联合照射组相比，S期比例差异无统计学意义（P〉 0.05）。结论 阿帕替尼能增加食管癌Kyse-150细胞的放射敏感性，其机制可能与抑制细胞增殖、促进细胞凋亡及诱导细胞周期再分布有关。
Objective To evaluate the radiosensitization effect of apatinib on esophageal cancer cell line Kyse-150, and to investigate the underlying mechanism. Methods Cells were divided into four groups：control group, apatinib treatment group, X-ray radiation group, and the combination group treated with X-rays plus apatinib. The effect of apatinib with different concentrations on the cell proliferative and radiosensitivity were evaluated by CCK-8 kit and colony formation assay. Flow cytometry method was adopted to detect the effect of apatinib on cell cycle progress and apoptosis induction. Results Apatinib inhibited the proliferation of Kyse-150 cells in time-and dose-dependent manners （r=0.89-0.96, P〈0.05）. With the increase of apatinib concentration, D0, Dq and SF2 value of Kyse-150 cells decreased and SERD0 value increased. Compared with control group, apatinib alone group, and radiation alone group, the cell apoptosis rate significantly increased in the combination group （t=12.36, 5.99, 15.47,P〈0.05）. Compared with control group, the percentages of cells in G2/M phase were all significantly increased in apatinib group, radiation group and combination group （t=8.81, 39.69, 20.61,P〈0.05）. Compared with radiation alone group and control group, the percentage of cells in S phase significantly increased in apatinib alone group and combination group（t=6.06, 3.82,8.81,6.24,P〈0.05）. ConclusionsApatinib can increase radiosensitivity of esophageal cancer cell line Kyse-150 possibly by inhibiting cell proliferation, inducing cell apoptosis and causing redistribution of cell cycle.
作者
孙菲
孙志强
汪建林
王坚
胡莉钧
周梦耘
封悦
孔泽
华秋
于静萍
Sun Fei;Sun Zhiqiang;Wang Jianlin;Wang Jian;Hu Lijun;Zhou Mengyun;Feng Yue;Kong Ze;Hua Qiu;Yu Jingping(Department of Radiotherapy, Changzhou No. 2 People＇s Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China)
出处
《中华放射医学与防护杂志》
CAS
CSCD
北大核心
2017年第11期805-809,831,共6页
Chinese Journal of Radiological Medicine and Protection
基金
国家自然科学基金（11705095）
