摘要
【目的】探讨桃叶鸦葱总黄酮对脑缺血再灌注小鼠脑组织损伤的修复作用及其机制。【方法】采用结扎右侧颈总动脉制备脑缺血再灌注小鼠模型。将96只清洁级雄性健康昆明种小鼠随机分成假手术组,模型组,桃叶鸦葱总黄酮高、中、低剂量组(剂量分别为400、200、100 mg·kg-1·d-1),依达拉奉组(剂量为6 mg·kg-1·d-1),每组16只。再灌注后72 h,各组小鼠进行神经功能损伤评分;实验结束后,采用氯化-2,3,5-三氯苯基四氮唑(TTC)染色法测定各组小鼠脑梗死范围,采用硫代巴比妥酸(TBA)法测定脑组织内丙二醛(MDA)含量,采用羟胺法测定超氧化物歧化酶(SOD)活性,采用5,5-二硫二硝基苯甲酸(DTNB)比色法检测谷胱甘肽过氧化物酶(GSH-Px)活性。【结果】与假手术组比较,模型组小鼠神经功能损伤评分明显增加,病理表现为脑梗死,脑组织内MDA含量明显升高,SOD、GSH-Px活性明显降低,差异均有统计学意义(P<0.01)。与模型组比较,桃叶鸦葱总黄酮高、中、低剂量组小鼠神经功能损伤评分明显减少,脑梗死面积明显缩小,脑组织内MDA含量明显降低,SOD、GSH-Px活性显著升高,均表现出明显的量效依赖关系;其中,桃叶鸦葱总黄酮高、中剂量组效果更明显(均P<0.05),且与依达拉奉组比较,差异无统计学意义(P>0.05)。【结论】桃叶鸦葱总黄酮对小鼠脑缺血再灌注损伤具有修复作用,可能与其具有抗氧化活性的功能有关。
Objective To study the effect of the total flavonoids of Scorzonera sinensis Lipsch（TFSSL） on repairing cerebral ischemia reperfusion injury in mice and to explore their mechanism. Methods The mice model of cerebral ischemia reperfusion was established by ligating the right common carotid artery. Ninety-six clean healthy male mice were divided into 6 groups,namely sham-operation group,model group,high-,middle-,low-dose TFSSL groups（400,200,100 mg·kg-1·d-1,respectively）,Edaravone group（6 mg·kg-1·d-1）,16 mice in each group. Seventy-two hours after cerebral ischemia reperfusion,mouse neurological severity scores in various groups were assessed. At the end of the experiment,the cerebral infarction size was measured by 2,3,5-triphenyl tetrazolium chloride（TTC） staining method, the content of malondialdehyde（MDA） in brain tissue was determined by thiobarbituric acid（TBA） method, superoxide dismutase（SOD） activity was observed by hydroxylamine method, and glutathione peroxidase（GSH-Px） activity was measured by 5,5＇-dithio-bis-2-nitrobenzoic acid（DTNB） method. Results Compared with the sham-operation group,the neurological severity scores were increased in the model group,the cerebral infarction was found,the cerebral content of MDA was enhanced, and the activities of SOD and GSH-Px were decreased in the brain tissue, the differences being statistically significant（P〈0.01）. Compared with the model group,the neurological severity scores in the three TFSSL groups were significantly decreased, the cerebral infarction area was reduced, the cerebral content of MDA was significantly decreased, and the activities of SOD and GSH-PX were increased, the effects being dose-dependent. High-,and middle-dose TFSSL groups had the stronger effect（P〈0.05）,and the difference was insignificant as compared with edaravone group（P〈0.05）. Conclusion TFSSL are effective on repairing the mice cerebral ischemia reperfusion injury,and the mechanism may have a relation with the antioxidant function.
出处
《广州中医药大学学报》
CAS
2017年第6期902-906,共5页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
河北省中医药管理局科研项目(编号:2015172)
关键词
桃叶鸦葱总黄酮
脑缺血再灌注
氧化反应
疾病模型
动物
小鼠
total flavonoids of Scorzonera sinensis Lipsch
cerebral ischemia reperfusion
oxidation
disease models, animal
mice
