摘要
以绒毛皂荚(Gleditsia vestita Chun et How ex B.G.Li)种子为外植体,通过愈伤组织诱导、不定芽分化增殖、生根和驯化移栽,建立了绒毛皂荚组织培养的快速繁殖体系。结果表明:1)绒毛皂荚子叶和胚轴在MS+6-BA3.0 mg/L+KT 0.4 mg/L+IAA 0.3 mg/L培养基上同步诱导出愈伤和芽,愈伤组织诱导率达到90.5%,同步分化率高达77.9%;2)不定芽增殖的最佳培养基为1/2MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+NAA 0.2 mg/L,培养20 d后,增殖倍数为4.0;3)MS+NAA 0.5 mg/L+IAA 0.25 mg/L条件下,生根率高达100%,且移栽后植株长势好,成活率达90%以上。
The seeds of Gleditsia vestita Chun et How ex B. G. Li were used as explant material. Through callus differentiation, adventitious bud multiplication, acclimatization and transplantation, the tissue culture system was set up. The results are as follows： 1） The best group appeared in the medium containing MS＋6- BA 3.0 mg/L＋KT 0.4 mg/L＋IAA 0.3 mg/L, in which the callus and differentiated adventitious buds were in- duced synchronously from the cotyledons and hypocotyls. The callus induction rate reached 90.5% and syn- chronous differentiation rate reached 77.9% ; 2） The average proliferation times of buds were 4.0 in the medium containing 1/2MS＋6-BA 1.0 mg/L＋IBA 0.3 mg/L＋NAA 0.2 mg/L; 3） The rooting rate was 100% in MS＋NAA 0.5 mg/L＋IAA 0.25 mg/L. The plantlets grew well with 90% survival after transplantation.
作者
田学辉
黄丽芳
周柳
吴尧晶
刘克明
TIAN Xue-hui HUANG Li-fang ZHOU Liu WU Yao-jing LIU Ke-ming(College of Life Sciences, Hunan Normal University, Changsha 410081, Hunan, China Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha 410125, Hunan, China)
出处
《生命科学研究》
CAS
CSCD
2017年第5期377-381,共5页
Life Science Research
基金
国家自然科学基金资助项目(30470130)
国家科技基础性研究专项(2013FY111500)
湖南省林木种质资源调查(湘财农指(2015)91号)
湖南省外来入侵生物调查(湘财农指(2014)91号)
湖南省农业野生植物资源调查(湖南省农业厅)
湖南省生态学重点学科建设项目(0713)
关键词
濒危物种
绒毛皂荚
种子
组织培养
正交设计
endangered species
Gleditsia vestita Chun et How ex B. G. Li
seed
tissue culture
orthogonaldesign
