摘要
目的:观察葛根素对人非小细胞肺癌A549细胞生长的抑制作用及其机制。方法:体外培养人非小细胞肺癌细胞(A549),不同浓度(60μg/ml,120μg/ml,240μg/ml)葛根素处理24 h后;采用CCK-8法观察葛根素对细胞的增值抑制作用;吖啶橙(AO)/溴化乙锭(EB)双染法及AnnexinⅤ-PI双染流式细胞术检测药物作用前后A549细胞的形态学变化及凋亡状况;Western blot法检测Apelin/APJ蛋白水平的变化。结果:CCK-8法检测结果说明葛根素能抑制A549细胞的增值,具有浓度和时间依赖关系;流式细胞术进一步证实葛根素具有诱导细胞凋亡的作用,与A549细胞组比较,葛根素各处理组Apelin/APJ蛋白水平均有不同程度下调。结论:葛根素可能通过调节Apelin/APJ蛋白的表达诱导A549细胞凋亡。
Objective： To explore the effect of puerarin induced apoptosis in human non-small cell lung canner ＋4549 cells and its possible mechanisms. Methods： A549 cells were cultivated in vitro. When 80% ＋ 90% confluence was reached, they were treated with puerarin in different concentration （60,120,240 μg/ml）. The effects on cell apoptpsis were examined by CCK-8 colorimetry, Characteristic morphological changes of apoptosis were observed by AO/EB staining. The apoptosis of .4549 ceils were detected by flow cytometry analysis. The proteins level of Apelin and APJ were tested by Western blot. Results. CCK-8 test showed that puerarin could inhibit the proliferation of A549 cells in a time and concentration dependent manner. Flow cytometry analysis indicated that puerarin could make A549 cells apoptosis. Compared with the A549 cells group, the protein expression of Apelin/APJ was decreased in puerarin groups. Conclusion： Puerarin induces apoptosis in A549 cells , and its mechanism may through the regulation of Apelin/APJ expression.
出处
《中国应用生理学杂志》
CAS
CSCD
2017年第5期466-469,I0002,共5页
Chinese Journal of Applied Physiology
基金
皖南医学院校级重点科研项目培育基金(WK2017Z04)
