摘要
目的诱导扩增BALB/C小鼠滑膜抗原特异性调节性T细胞(antigen specific regulatory T cells,sTregs)的产生,为探索滑膜抗原sTregs在类风湿性关节炎(rheumatoid arthritis,RA)的治疗作用提供实验基础。方法体外诱导培养小鼠骨髓源性树突状细胞(DCs)并负载Ⅱ型胶原蛋白肽,在白细胞介素-2(IL-2)的协同作用下,与小鼠脾CD4^+CD25^+自然发生的调节性T细胞(nTregs)混合培养,以刺激产生滑膜抗原sTregs,并用混合淋巴细胞抑制实验评估扩增的sTregs抑制CD4^+CD25^-T细胞增殖的功能是否具有抗原特异性。结果经DCs刺激1周后,小鼠CD4^+CD25^+Tregs扩增了6倍,并且增殖的sTregs在混合淋巴细胞抑制试验中较多克隆Tregs(Poly-Tregs)具有较强的抑制作用(P<0.01),显示抗原特异性抑制的特点。结论以自身DCs负载滑膜源性抗原(Ⅱ型胶原蛋白肽)可以从小鼠脾CD4^+CD25^+nTregs中诱导扩增滑膜抗原sTregs。
Objective To amplify the production of synovium specific regulatory T cells in mice （ sTregs）, and to provide experimental basis for exploring the therapeutic effect of sTregs on Rheumatoid arthritis （RA）. Methods Mouse hone marrow derived DCs were induced in vitro and loaded with type Ⅱ collagen peptide, subsequently co - cultured with mouse spleen CD4^＋ CD25^＋ nTregs under the synergistic action of IL - 2 to stimulate the production of synovial antigen sTregs. The inhibition effect of sTregs was evaluated by mixed lymphocyte inhibition assay. Results After the stimulation of DCs for a week, mouse CD4^＋ CD25^＋ Tregs were amplified by 6 times, and the proliferating ceils （sTregs） showed sig- nificantly stronger inhibition in mixed lymphocyte inhibition test than Poly - Tregs （P 〈0.01 ）. Conclusion The Synovial antigen sTregs could be induced by the DCs loaded with synovial antigen （Type 11 collagen peptide） from mouse spleen CD4^＋ CD25^＋ nTregs.
出处
《广东医学》
CAS
北大核心
2017年第19期2907-2912,共6页
Guangdong Medical Journal
基金
广东省科技计划项目(编号:2013B021800239)
