摘要
目的探讨microRNA-96-5p对胃癌细胞哺乳动物雷帕霉素靶蛋白(the mammalian target of rapamycin,mTOR)表达的影响。方法利用慢病毒表达载体LV-hsa-mir-96-5p转染BGC-823胃癌细胞株。qRT-PCR检测胃癌细胞转染后miR-96-5p及mTOR mRNA的表达变化;Western blot检测mTOR蛋白表达的变化。结果病毒转染72 h后,实验组细胞miR-96-5p表达丰度是空白对照组的20.4倍(P<0.05),同时mTOR基因表达丰度下调至空白对照组的0.47倍(P<0.05),且mTOR蛋白的相对表达量与空白对照组相比明显下调(P<0.05)。结论胃癌细胞miR-96-5p的过表达可以下调mTOR基因mRNA及蛋白的表达,因此mTOR可能是miR-96-5p的下游靶基因之一。
Objective To investigate the effect of microRNA-96-5p （miR-96-5p） on expression of mam- malian target of rapamyein （mTOR） in gastric cancer cells. Methods Lentiviral vector, LV-hsa-mir-96-5p was transfected into BGC-823 gastric cancer cells. The expression of miR-96-5p and mTOR mRNA after transfeetion was detected by qRT-PCR, and the expression of mTOR protein was detected by Western blot. Results At 72 h after transfection, the expression level of miR-96-5p in the experimental group was 20.4 times of that in the blank control group （P 〈 0.05 ）, while the expression level of mTOR gene was downregulated to 0.47 times of that in the blank control group （P 〈 0.05） , and mTOR protein relative expression was significantly reduced compared with the control group （P 〈 0.05）. Conclusion Overexpression of miR-96-5p can downregulate the expression of mRNA and protein of mTOR gene in gastric cancer cells, thus, mTOR may be one of the downstream target genes of miR-96-5p.
出处
《实用医学杂志》
CAS
北大核心
2017年第18期2991-2994,共4页
The Journal of Practical Medicine
基金
贵州省科学技术基金项目(编号:[2015]2095)
贵州省卫计委科学技术基金项目(编号:gzwjkj2015-1-019)
