摘要
运用酶活性染色和酶抑制动力学方法分析了α-溴代肉桂醛对蘑菇酪氨酸酶的抑制作用,测得酶的正向和逆向速率常数分别为2.793L/(mmol·min)和1.98×10-3 s-1.在此基础上探讨了α-溴代肉桂醛对棉铃虫(Helicoverpa armigera)的毒杀作用,发现经0.625mmol/Lα-溴代肉桂醛处理后,棉铃虫3龄幼虫的生长发育受到一定程度限制,当α-溴代肉桂醛浓度达5.0mmol/L时,在作用48h后致死.此外,经5.0mmol/Lα-溴代肉桂醛处理后,棉铃虫3龄幼虫的活体和离体多酚氧化酶(PPO)活力受到了显著抑制.上述结果为今后α-溴代肉桂醛在食品、农业、医药等方面的应用提供了理论和实践基础.
The inhibitory effect ofα-bromocinnamaldehyde on mushroom tyrosinase was investigated in this study using enzyme activity staining and enzyme inhibitory kinetics.The rate constants of the forward（k＋0）and reverse（k-0）reactions were determined to be 2.793L/（mmol·min）and 1.98×10^-3 s^-1,respectively.Moreover,the insecticidal efficacy ofα-bromocinnamaldehyde was determined.As expected,the larval growth and survival rate of Helicoverpa armigera were found to be reduced after supplemented with 0.625mmol/Lα-bromocinnamaldehyde as compared to the control larvae,and the larvae were totally dead after 48 htreatment with 5.0mmol/Lα-bromocinnamaldehyde.The polyphenol oxidase（PPO）activities were inhibited by 5.0mmol/Lα-bromocinnamaldehyde both in vivo and in vitro.These results may provide theoretical and practical basis in the application ofα-bromocinnamaldehyde in food,agriculture and medicine.
出处
《厦门大学学报（自然科学版）》
CAS
CSCD
北大核心
2017年第5期659-664,共6页
Journal of Xiamen University：Natural Science
基金
国家自然科学基金(31271952)
关键词
α-溴代肉桂醛
酪氨酸酶
棉铃虫
α-bromocinnamaldehyde
tyrosinase
Helicoverpa armigera
