摘要
目的:研究复方芒果叶对博莱霉素诱导小鼠肺纤维化的作用。方法:建立博莱霉素诱导小鼠肺纤维化模型,观察复方芒果叶对模型动物整体行为学,HE、Masson染色后的组织病理学,以及检测肺指数、羟脯氨酸、丙二醛、还原型谷胱甘肽、超氧化物歧化酶、总抗氧化能力、炎症因子IL-1β、IL-6、TNF-α的表达水平。结果:复方芒果叶18.0 g/kg、9.0 g/kg及4.5 g/kg剂量组均可显著性降低肺泡炎和纤维化程度,降低肺指数以及羟脯氨酸、丙二醛、IL-6、TNF-α含量,同时,显著性升高超氧化物歧化酶含量和总抗氧化能力,复方芒果叶18.0 g/kg和9.0 g/kg剂量组均可明显改善肺纤维化小鼠整体行为学,升高还原型谷胱甘肽含量,复方芒果叶18.0 g/kg和4.5 g/kg剂量组均可显著性降低IL-1β含量。结论:复方芒果叶可减轻博莱霉素诱导肺纤维化小鼠的纤维化程度,降低其炎症和氧化水平,具有良好的抗炎、抗氧化、抗纤维化作用。
Objective： To investigate the effect of Compound Mango Leaves on mice with bleomycin-induced pulmonary fibrosis. Methods： we established the model of pulmonary fibrosis by using bleomycin to observe the behavior of mice, histopathology in HE, Masson staining and to detect the levels of lung index, HYP, MDA, GSH, SOD, T-AOC, IL-1β, IL-6, TNF-α. Results： Compound Mango Leaves（18.0g/kg, 9. 0g/kg,4.5g/kg） could obviously reduce the degrees of pulmonary alveolitis and fibrosis, the levels of lung index, HYP, MDA, IL-6, TNF-α. Meanwhile, it also obviously increased the levels of SOD and T-AOC. Compound Mango Leaves（ 18.0g/kg and 9.0g/kg） could obviously improve the behavior and increased the levels of GSH. Compound Mango Leaves（ 18. 0g/kg and 4.5g/kg） could obviously reduce the levels of IL-1β. Conclusion： Compound Mango Leaves could relieve the degree of Bleomyein-induced pulmonary fibrosis in mice, reduce the levels of inflammation and oxidation, and had a good effect on anti-inflammatory, antioxidant, anti-fibrosis.
出处
《中药药理与临床》
CSCD
北大核心
2017年第3期123-127,共5页
Pharmacology and Clinics of Chinese Materia Medica
基金
广西科技基础条件平台项目(10-046-04)
广西"八桂学者"工程专项经费资助(厅发[2013]3号)
关键词
复方芒果叶
肺纤维化
博莱霉素
小鼠
Compound Mango Leaves （复方芒果叶）
Pulmonary fibrosis
Bleomycin
