摘要
利用豆制品生产过程中的废水(黄浆水)对枯萎病拮抗菌Pb-4菌株的发酵培养基及其发酵条件进行优化。通过单因素试验筛选Pb-4菌株黄浆水培养基中的碳源;设置因素水平为L_(18)(3~5)正交试验,对Pb-4菌株黄浆水培养基中最佳碳源和无机盐成分进行筛选和优化,确定发酵培养基配方;通过响应面优化试验对Pb-4菌株的发酵温度、p H和接种量进行优化。结果显示,通过筛选确定玉米粉为黄浆水培养基的最佳碳源;优化后的发酵培养基配方为:1 000 m L黄浆水中添加玉米粉30 g、(NH_4)_2SO_4 2g、Mg SO_4 1.5 g、CaCO_3 1.5 g和FeSO_4 0.3 g;优化后的发酵条件为:温度36.3℃、p H7.4、接种量5%、摇瓶培养40 h。在此优化条件下,发酵菌数可达42.8×10~8 CFU/m L、最大生物量OD_(660)值为0.393,与预测理论最大值0.390 2相吻合。优化后的发酵培养基能够替代蛋白胨、酵母粉和葡萄糖等常规培养基,具有简单、廉价和环保等特点,可作为微生物菌剂规模化生产的原料。
The wastewater from soybean processing（yellow serofluid）was used to optimize the fermentation medium condition ofantagonistic bacterium Pb-4 against fusarium wilt. The single factor experiments were conducted to screen the carbon source of Pb-4 strain inyellow serofluid medium. Orthogonal experiments L18（35）were designed to select the optimal carbon source and inorganic components forPb-4 strain in yellow serofluid medium,and determine the optimal fermentation medium formulation. The fermentation temperature,p H andinoculation amount of Pb-4 strain were optimized by response surface method（RSM）. As results,the composition of the optimal mediumwas yellow serofluid 1 000 m L,corn flour as the optimal carbon source 30 g,（NH_4）_2SO_4 2 g,MgSO_4 1.5 g,CaCO_3 1.5 g and FeSO_4 0.3 g.The fermentation conditions after optimization were as ：temperature 36.3℃,inoculum size 5%,p H 7.4 and incubation time 40 h. Under theoptimized conditions,the number of fermented bacteria was up to 42.8×10~8 CFU/m L,and the maximum biomass OD_（660） was 0.393,whichwas in accord with the predicted value 0.390. In conclusion,the optimized medium can be as an effective substitute for traditional mediumlike peptone,yeast powder,glucose,etc.,moreover as it is simple,cost-efficient and environmentally friendly,thus it can be used as rawmaterial for the scale production of microbial agents.
出处
《生物技术通报》
CAS
CSCD
北大核心
2017年第8期167-173,共7页
Biotechnology Bulletin
基金
山西省煤基重点科技攻关项目(FT201402-15)
山西省专利推广实施资助专项(20161016)
关键词
黄浆水
解淀粉芽孢杆菌
番茄枯萎病菌
拮抗菌株
发酵条件优化
yellow serofluid
Bacillus amyloliquefaciens
Fusarium oxysporum f
sp
lycopersiici
antagonistic bacteria
fermentation condition optimization
