摘要
目的探索长链非编码RNA(long intergenic non-coding RNA,lincRNA)-EPS在非小细胞肺癌(nonsmall-cell lung cancer,NSCLC)组织中的表达及与临床病理特征的关系。方法采用原位杂交的方法检测lincRNAEPS在NSCLC组织和癌旁组织中表达定位。定量PCR方法检测lincRNA-EPS在NSCLC组织和癌旁组织中的表达水平,分析其与NSCLC临床病理特征的关系。结果 LincRNA-EPS在NSCLC组织中高表达率为71.4%,高于癌旁组织(P<0.01)。LincRNA-EPS在TNMⅢ/Ⅳ期和有淋巴结转移的NSCLC组织中的表达水平高于Ⅰ/Ⅱ期和无淋巴结转移的组织(均P<0.05)。在中低分化与高分化的NSCLC组织中lincRNA-EPS表达比较差异无统计学意义(P>0.05)。结论 NSCLC中lincRNA-EPS的表达与临床分期和淋巴结转移有关。
Objective To investigate the expression of long chain non-coding RNA-EPS（ lincRNA-EPS） in nonsmall-cell lung cancer（ NSCLC） and to explore its relationship with the clinicopathological characteristics of the disease.Methods To examine the expression and localization of lincRNA-EPS in NSCLC tissues and adjacent normal lung tissues by in-situ hybridization. The expression level of lincRNA-EPS in NSCLC and adjacent normal tissues was tested by quantitative polymerase chain reaction. The association between lincRNA-EPS expression and the clinicopathological characteristics of NSCLC was further analyzed. Results The high expression rate of lincRNA-EPS in NSCLC tissues was 71. 4%,which was significantly higher than that in adjacent normal lung tissues（ P〈0. 01）. Moreover,lincRNA-EPS level was significantly higher in NSCLC tissues of TNM Ⅲ/Ⅳ stages or with lymph node metastasis,than in NSCLC tissues of TNM Ⅰ/Ⅱ stages or without lymph node metastasis（ both P〈0. 05）. Nevertheless,the expression of lincRNA-EPS was comparable between NSCLC tissues of low-medium differentiation and those of high-differentiation（ P〉0. 05）. Conclusion The expression of lincRNA-EPS is related with the clinical stages and lymph node metastasis of NSCLC.
作者
张云嵩
蔡迎玖
张彦伟
董建林
白芳
高小俊
陈田子
Zhang Yunsong Cai Yingjiu Zhang Yanwei(Department of Thoracic Surgery,Tianjin University of Traditional Chinese Medicine Affiliated Wuqing Traditional Chinese Medicine Hospital, Tianjin 301700, China)
出处
《实用肿瘤杂志》
CAS
2017年第4期349-351,共3页
Journal of Practical Oncology
