摘要
目的:观察白花蛇舌草提取物对人前列腺癌DU145细胞增殖和凋亡的影响,并初步探讨其作用机制。方法:不同浓度(0.25 g·L-1、0.5 g·L-1、1 g·L-1)白花蛇舌草提取物作用人前列腺癌DU145细胞24 h、48 h、72 h后,使用CCK-8法检测白花蛇舌草提取物对DU145细胞增殖的影响;流式细胞术检测细胞凋亡的改变;实时定量PCR检测COX-2 m RNA和PCNA m RNA的表达;Western bloting法检测Bcl-2、Bax、Caspase3、Cyclin D1蛋白表达。结果:随着作用时间的延长和剂量的增加,白花蛇舌草提取物对DU145细胞增殖抑制率明显提高,呈时效和量效依赖效应。白花蛇舌草提取物作用48 h后,可明显提高细胞凋亡率,下调DU145细胞COX-2 m RNA、PCNA m RNA及Bcl-2、Cyclin D1蛋白表达,上调Bax、Caspase3蛋白表达,呈量效依赖效应。结论:白花蛇舌草提取物能降低人前列腺癌DU145细胞增殖能力,并诱导其凋亡,其机制可能与改变细胞周期和影响凋亡相关基因表达有关。
Objective：To investigate the effect of Hedyotis diffusa extract on proliferation and apoptosis of prostate cancer cell line DU145 and its possible mechanisms. Methods：Prostate cancer cell line DU145 were treated with different concentration of Hedyotis diffusa extract（0. 25 g·L^-1,0. 5 g·L^-1,1 g·L^-1） for 24 h,48 h,72 h,CCK-8 assay was used to detect the proliferation of DU145 cell. Flow cytometry was performed to detect cell apoptosis;RT-PCR was carried out to detect the m RNA expression of COX-2 and PCNA;Western bloting was used to examine the protein expression of Bcl-2,Bax,Caspase3 and Cyclin D1. Results：The inhibited effect of Hedyotis diffusa extract in DU145 proliferation exhibited dose and time dependent manner. The concentration of Hedyotis diffusa extract treated longer than 48 h could inhibit the proliferation of DU145 cells,the COX-2 m RNA,PCNA m RNA and Bcl-2,Cyclin D1 expression of DU145 cells was significantly decreased,Bax and Caspase3 expression significantly increased,the change degree was in a dose-dependent manner. Conclusion： Hedyotis diffusa extract can inhibit the proliferation of DU145 cells and induce the apoptosis of DU145 cells,which may occour though change the expression of cell cycles and apoptosis-related protein.
出处
《中医学报》
CAS
2017年第6期914-917,共4页
Acta Chinese Medicine
基金
上海市卫生和计划生育委员会项目(ZXBZ2012-07)
上海市卫生和计划生育委员会中医药科研专项课题(2016JP014)
上海中医药大学预算内课题基金项目(2014Y3N38)
