摘要
目的:研究纤维素酶法提取谷精草中总黄酮的最佳工艺。方法:采用紫外分光光度法分别考察酶用量、提取时间、料液比、提取温度对谷精草总黄酮提取率的影响,并在此基础上设计L9(34)正交试验,优选最佳提取工艺。结果:最佳条件是酶用量0.03 g,提取时间2.5 h,料液比1∶40,提取温度55℃,采用最佳工艺,总黄酮提取率可达0.879%。结论:该方法可以用于谷精草总黄酮的提取。
Objective：To study the optimum extraction technology of total flavonoids from Eriocaulon buergeria- Mum Koern by cellulase method. Methods： UV spectrophotometric method was used to observe the influence of enzyme dosage, extraction time, extraction temperature and solid - liquid ratio on the extraction rate of total fla- vonoids from Eriocaulon buergerianum Koern. And on this basis, the L9 （34） orthogonal test had been used to select the best extraction. Results： The optimum condition was enzyme dosage 0.03 g, extraction time 2.5 h, material liquid ratio 1：40, extraction temperature 55℃, and the extraction rate of flavonoids was 0. 879% by using the optimum condition. Conclusions： The method can be used for the extraction of total flavonoids from Eriocaulon buergerianum Koern.
出处
《安徽科技学院学报》
2017年第1期60-64,共5页
Journal of Anhui Science and Technology University
基金
安徽省教育厅自然科学重点课题(KJ2016A500)
亳州市中药科技创新团队(BZ2013zzb06)
关键词
谷精草
纤维素酶
总黄酮
提取工艺
Eriocaulon buergerianum Koern
Cellulase
Total flavonoids
Extraction technology
