摘要
【目的】研究不同采摘时间鲜芍药花精油的化学成分及其体外抗氧化活性,为适时采收、合理开发及利用芍药花资源提供科学依据。【方法】采用水蒸气蒸馏法提取日出前(06:00-07:00)和下午(17:00-18:00)采摘的芍药花精油,通过气相色谱-质谱联用技术对其化学成分进行分析鉴定,采用峰面积归一化法确定各组分的相对含量;测定质量浓度为20,50,100,150和200μg/mL芍药花精油溶液对DPPH自由基、ABTS自由基的清除效果,及对亚油酸脂质过氧化的抑制作用。【结果】从采摘于日出前的芍药花精油中共鉴定出35种化学组分,占精油总量的98.85%,主要成分为正二十五烷(21.14%)、正二十九烷(10.52%)、正二十三烷(9.30%)、棕榈酸(9.14%)、亚油酸(8.43%)、正二十四烷(6.74%)、3-十二烷基-2,5-呋喃二酮(4.13%)、正二十一烷(3.58%)、金合欢基丙酮(3.45%)、正二十六烷(3.21%)、香叶基芳樟醇(2.78%)等。从采摘于日出后经日晒的芍药花精油中共鉴定出27种化学组分,占精油总量的98.10%,主要成分为正二十五烷(22.54%)、棕榈酸(11.81%)、正二十七烷(10.27%)、正二十三烷(8.28%)、六氢金合欢基丙酮(8.80%)、金合欢基丙酮(7.35%)、正二十四烷(6.07%)、正二十六烷(3.96%)、正二十一烷(3.90%)、β-4,8,13-杜法三烯-1,3-二醇(3.40%)等。精油体外抗氧化活性结果表明,芍药花精油对DPPH自由基和ABTS自由基具有一定的清除能力,同时对亚油酸脂质过氧化具有一定的抑制作用,且体外抗氧化作用随着精油质量浓度的增大而增强。【结论】芍药花精油化学成分较丰富,且具有较好的体外抗氧化活性,在食品香料和医药方面具有较高的开发利用价值。
[Objective] The chemical composition and antioxidant activities of essential oil from Paeonia lactiflora Pall. flowers collected at different times were determined. [Method]The essential oil was ex- tracted from P. lactiflora flowers by hydro distillation and the chemical composition was analyzed by gas chromatography-mass spectrometry （GC-MS）. The relative contents were determined by peak area normalization method and the antioxidant activities were evaluated based on scavenging 2,2-diphenyl-l-picryl- hydrazyl（DPPH） radical, 2,2＇-azino-bis （3-ethylbenzothiazoline-6-sulfonic acid） di-ammonium salt （ABTS） radical,and inhibition of lipid peroxidation in linoleic acid. [Result] Thirty-five compounds accounting for 98.85% of the essential oil were obtained from flowers collected before sunrise,and major components in- cluded pentacosane （21.14%）,octacosane （10.52%）, tricosane （9.30%）, palmitic acid （9.14%）, linoleic acid （8.43%）, tetracosane （ 6. 74%）, 3-dodecyl-2,5-furandione （ 4. 13%）, heneicosane （ 3.58 % ）, farnesyl acetone （3. 45%）, hexacosane （3. 21%）, and geranyllinalool （2. 78%）. Twenty-seven compounds of 98.10% were obtained from flowers collected after sunrise. The main compounds were pentacosane （22.540%）, palmitic acid （11.81%）, octacosane （ 10.27 %）, tricosane （8.28%）, hexahydrofarnesyl acetone （8.80%） ,farnesyl acetone （7.35%） ,tetracosane （6.07%）, hexacosane （3.96%）, heneicosane （3.90%）, and 13-4,8,13-duvatriene-1,3-diol （3.40%）. The essential oil had strong scavenging effects against DPPH free radical and ABTS free radical,＇and inhibited the lipid peroxidation of linoleic acid. The anti-oxidation a- bility increased with the increase of essential oil concentration. [Conclusion] The essential oil form P. lac- tiflora flowers were rich in chemical components and had high antioxidant activities. Thus,it is a natural potential source of preservative for food and other allied industries.
出处
《西北农林科技大学学报（自然科学版）》
CSCD
北大核心
2017年第5期204-210,共7页
Journal of Northwest A&F University(Natural Science Edition)
基金
国家民委科研项目(14BFZ005)
陕西省科技计划项目(S2014SF4025)
河西学院青年教师基金项目(QN2014-20)
关键词
芍药花
精油
气相色谱-质谱法
抗氧化活性
Paeonia lactiflora Pall flowers
essential oil
GC-MS
antioxidant activity
