摘要
目的通过将骨髓间充质干细胞复合Ⅰ型胶原修饰的聚乳酸聚乙醇酸(PLGA)微球支架注入骨质疏松大鼠股骨转子间,探讨其对骨缺损后骨质量的局部改善情况。方法通过切除30只SD雌性大鼠双侧卵巢建立骨质疏松动物模型,并用电钻在股骨转子间人为制作骨缺损;实验动物按随机数字表法均分为给药组、模型组和假手术组。给药组大鼠骨缺损部位注入骨髓间充质干细胞复合Ⅰ型胶原修饰的PLGA微球,模型组大鼠骨缺损部位注入PBS缓冲液,假手术组大鼠行假手术。分别考察术后1、3个月骨缺损部位的骨矿含量、骨密度、骨小梁厚度、骨小梁面积百分比及分离度。结果骨髓间充质干细胞与Ⅰ型胶原修饰的PLGA微球支架体外培养7 d,发现细胞在支架表面黏附、生长良好。与模型组比较,给药组动物术后1个月的骨密度、骨小梁厚度、骨小梁面积百分比均显著增大,骨小梁分离度显著减小,均差异有统计学意义(P<0.05),骨矿含量变化不明显(P>0.05);给药组动物术后3个月的骨矿含量、骨密度、骨小梁厚度、骨小梁面积百分比均显著大于模型组,骨小梁分离度显著小于模型组,均差异有统计学意义(P<0.05)。结论骨髓间充质干细胞复合Ⅰ型胶原修饰的PLGA微球可修复大鼠骨质疏松部位骨缺损,改建骨小梁结构,提高骨质量。
Objective To study the effects of PLGA/CoI microspheres combined with BMSCs on bone defects in animal model with osteoporosis. Methods Thirty Sprague Dawley rats were ovariectomized to establish osteoporosis animal models, and the intertrochan-teric bone defect was made with electric drill. The models were randomly assigned into sham group （treated with sham operation） ,con-trol group （the intertrochanteric bone defect sites were treated with phosphate buffer saline） and drug administration group （the inter-trochanteric bone defect sites were treated with PLGA/CoI microspheres combined with BMSCs） . The bone mineral content,bone miner-al density, trabecular thickness, percentage of trabecular area and trabecular spacing after operaton for 1,3 months were compared. Re-sults After 7 days of coculture, BMSCs seeded on PLGA/CoI microspheres had nice adherence and proliferation. The bone mineral density,trabecular thickness and percentage of trabecular area were larger,the trabecular spacing value was smaller in drug administra-tion group compared with the control group after operation for one month （all P 〈0. 05） , respectively; the change of bone mineral con-tent was insignificant （P 〉0. 05 ） . Compared with the control group,the bone mineral content,bone mineral density, trabecular thickness and percentage of trabecular area increased significantly in drug administration group （all P 〈 0 .05 ） ; the trabecular spacing value de-creased significantly in drug administration group （P〈0. 05） . Conclusions The bone defect of osteoporotic site can be repaired,the trabecular reconstruction and bone quality of osteoporotic site also can be improved after the injection of the PLGA/CoI microspheres combined with BMSCs.
出处
《安徽医药》
CAS
2017年第4期618-622,共5页
Anhui Medical and Pharmaceutical Journal
关键词
骨髓间充质干细胞
聚乳酸聚乙醇酸
骨质疏松
骨密度
Bone mesenchymal stem cell
Ploylactic acid-polyglycolic acid copolymer
Osteoporosis
Bone mineral density
