摘要
为全面深入了解CRISPR在致病菌中的最新进展,该文简介了CRISPR的结构与功能,深入论述其在各类致病菌中的分型研究及应用情况,剖析CRISPR分型研究现有问题和措施,并展望今后CRISPR重点研究方向。CRISPR分型在各类致病菌中有着不同程度应用,在结核分枝杆菌复合群、沙门氏菌、产志贺毒素大肠杆菌等致病菌中已获成功。加强多种致病菌的全基因组测序及扩充CRISPR数据库,CRISPR分型将会应用更广泛。
In order to know the up - to - date progress of CRISPR in pathogens, the structure and function of CRISPR are briefly introduced,the study and application of CRISPR subtyping in various pathogens are discussed extensively, the current CRISPR subtyping associated issues are analyzed, and the prospective CRISPR research areas are discussed. The application of CRISPR subtyping varies in different pathogens, and it has been applied successfully in pathogens like Mycobacterium tuberculo- sis complex,Salmonella and Shiga toxin - producing Escherichia coli, etc. In order to achieve its extensive subtyping role, the whole genomes of various pathogens should be sequenced,and the CRISPR database should be expanded.
出处
《生物技术》
北大核心
2017年第2期186-191,共6页
Biotechnology
基金
国家重点研发计划项目("跨境食品潜在
新发病原微生物筛查监控技术及溯源平台建设"
No.2016YFD0401102)
江苏出入境检验检疫局青年基金项目("特殊位点序列分析在食源性致病菌中的研究及应用"
No.2016KJ49
"弯曲杆菌高通量同步检测分型技术研究及检测试剂盒AOAC认证"
No.2016KJ53)
质检总局科研项目("食品中弯曲杆菌多重实时荧光PCR检测分型技术研究及应用"
No.2016IK308)
关键词
成簇规律间隔短回文重复序列
分子分型
致病菌
clustered regularly interspaced short palindromic repeat sequences,molecular subtyping, pathogen
