摘要
目的对地衣抗癌活性部位AMH-T进行常规毒理学试验研究,了解其毒作用的性质和强度,为抗癌药物的开发提供安全性依据.方法急性毒性试验、小鼠骨髓微核试验、小鼠精子畸形试验、Ames试验.结果雄性小鼠经皮下注射给药的LD50为147 mg/kg,雌性小鼠的LD50为171 mg/kg.小鼠骨髓微核试验、小鼠精子畸形试验和Ames试验在试验剂量下未见阳性结果.结论 AMH-T皮下注射给药具有较强的急性毒性和肝毒性,实验剂量范围内未见遗传毒性.
Objective To provide safety reference for the development of anti-cancer drugs by evaluating thetoxicological safety of the anticancer bioactive fraction AMH-T of lichen through the understanding of its poisonous nature and the intensity. Methods Acute toxicity test, bone marrow micronucleus test in mice, sperm malformation test in rats, Ames test and short-term repeat drug test in mice were conducted. Results Male mice were injected LD50 of 147 mg/kg and female mice 171 mg/kg. Conclusion Injection of AMH-T has acute toxicity and liver toxicity,but has no genetic toxicity.
作者
贺云发
贺小琼
刘敏
王静
胡松杉
税靖霖
姜重阳
HE Yun-fa HE Xiao-qiong LIU Min WANG Jing HU Song-shan SHUI Jing-ling JIANG Chong-yang(） School of Public Health, Kunming Medical University, Kunming Yunnan 650500 ） Yunnan Center for Disease Prevention and Control, Kunming Yunnan 650000, China)
出处
《昆明医科大学学报》
CAS
2016年第9期26-30,共5页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(30960437)
云南省科技计划基金资助项目(2009FXL001)
云南省科技厅-昆明医科大学应用基础研究联合专项基金重点资助项目(2012FB001)
关键词
地衣
抗癌药物
急性毒性
遗传毒性
毒理学
Lichen
Anti-cancer drug
Acute toxicity
Genotoxicity
Toxicology
