摘要
目的探讨一枝蒿挥发油抗炎、抗过敏的作用。方法将昆明种小鼠按体质量、雌雄随机分为模型组、一枝蒿挥发油高和低剂量组及滴通鼻炎水组,除模型组小鼠给予生理盐水滴鼻双侧以外,其余各组小鼠滴鼻给药,1h后以二甲苯涂耳致炎,通过小鼠耳肿胀抑制率考察一枝蒿挥发油抗炎作用。将昆明种小鼠按体质量、性别随机分为模型组、一枝蒿挥发油高和低剂量组及滴通鼻炎水组,除模型组、滴通鼻炎水组小鼠给予生理盐水外,其余各组均滴鼻给药,给药2次,于末次给药0.5h后,尾静脉注入伊文思蓝生理盐水溶液及腹腔注射冰醋酸溶液,收集腹腔生理盐水冲洗液并测定其吸光度值,考察一枝蒿挥发油对小鼠腹腔毛细血管通透性的影响。将昆明种小鼠随机分为正常对照组、模型组及一枝蒿挥发油高、低剂量组,于小鼠背部,除正常对照组给予4∶1丙酮食用油以外,其余小鼠涂以2,4-二硝基氯苯(DNCB)液(4∶1丙酮食用油液配制)致敏,除正常对照组和模型组给予生理盐水,其余各组小鼠滴鼻给药,考察一枝蒿挥发油对小鼠耳肿胀度及胸腺、脾脏系数的影响。将SD大鼠除正常对照组以生理盐水溶液双侧滴鼻外,其余大鼠于左右后肢足跖皮下、腹腔及两侧腹股沟皮下多点注射以氢氧化铝为佐剂的卵清蛋白(OVA)致敏混悬液。制备大鼠过敏性鼻炎(AR)模型,模型成功大鼠随机分为模型组、一枝蒿挥发油高和低剂量组和滴通鼻炎水组,除正常对照组和模型组大鼠给予赋形剂,其余大鼠双侧滴鼻相应药液,同时隔日用OVA生理盐水溶液滴鼻维持过敏状态,给药结束后,采用ELASA法测定大鼠血清中IgE、sIgE、IL-4的含量,考察一枝蒿挥发油抗过敏作用。结果与模型组小鼠比较,一枝蒿挥发油高剂量组小鼠的耳肿胀抑制为36.32%,一枝蒿挥发油低剂量组小鼠耳肿胀抑制率为34.53%,差异有统计学意义(P<0.05)。与模型组小鼠比较,一枝蒿挥发油高、低剂量组小鼠腹腔毛细血管通透性的增加均有抑制作用,抑制率分别为34.04%、30.82%,差异有统计学意义(P<0.05)。在DNCB迟发性超敏实验中,与模型组小鼠比较,比给药组小鼠超敏反应可缓解,抑制率可达35.20%,差异有统计学意义(P<0.05)。与模型组大鼠比较,过敏性鼻炎大鼠血清IL-4、IgE及sIgE含量下降,差异有统计学意义(P<0.05),与滴通鼻炎水组比较,则差异无统计学意义(P>0.05)。结论一枝蒿挥发油有较明显的抗炎、抗过敏作用。
Objective To evaluate the effect of Artemisia volatile oil on allergy and acute inflammation. Methods KM mice were randomly divided into model group, Artemisia volatile oil with high and low dose group and Ditong rhinitis water group, according to body weight and sex. Except of the model group that was given saline nasal side, the remaining mice administered. After 1 h, in ear inflammation induced by xylene coating, to investigate the anti-inflammatory effect of ear swelling inhibition with Artemisia volatile oil. KM mice were randomly divided into model group, Artemisia volatile oil of high and low dose group and Ditong rhinitis water group, according to body weight and sex. Except of the model group and Ditong rhinitis water group were given saline nasal side, the remaining mice administered. Administered 2 times, giving an intravenous injection of Evans blue saline and an intraperitoneal injection of acetic acid, collecting physiological saline solution and measuring the absorbance value, to investigate the effects of Artemisia volatile oil on mouse peritoneal capillary permeability. KM mice were randomly divided into model group, normal control group Artemisia volatile oil of high and low dose group. In addition to the normal control group was given 4 to 1 acetone edible oil, the other mice were coated with 2 4-, two nitrochlorobenzene （DNCB） solution （4 ： 1 acetone solution of edible oil） sensitization. In addition to the normal control group and model group were given normal saline, the rest mice administered, to investigate the effects of Artemisia volatile oil on the mouse ear swelling and thymus and spleen coefficient. SD rats except the nor- mal control group with saline solution of bilateral nasal drops, gave injection on the left and right hind paw subcutaneous and intraperitoneal and bilateral inguinal subcutaneous of adjuvant of ovalbumin （OVA） sen- sitized with aluminum hydroxide suspension of the rest rats. Preparation of allergic rhinitis rats （AR） model, the successful model rats were randomly divided into model group, Artemisia volatile oil of high and low dose group and the Ditong rhinitis water group. In addition to the normal control group and model group rats were treated with excipient, the rest rats bilateral nasal septum and corresponding liquid medi- cine, daily maintain nasal allergy status with the OVA saline solution. After medication, determination of the serum of IgE, sIgE and IL-4 was performed using ELASA methodto investigate effect of Artemisia volatile oil on the anti-allergic. Results Compared with the model group, the volatile oil of Artemisia in high dose group was shown to inhibit the ear swelling of 36.32% rupestonic volatile oil in low dose group mice ear swelling inhibition rate was 34.53%, the difference was statistically significant （P 〈0.05）. Com- pared with the model group mice, Artemisia volatile oil of high and low dose of mouse peritoneal capillary permeability increase were inhibited, the inhibition rates were 34.04%, 30. 82% respectively, the difference was statistically significant P 〈0.05. Compared with the model group, the mice in the DNCB delayed hypersensitivity test showed that the sensitivity of the mice could be reduced, the inhibition rate was 35.20%, and the difference was statistically significant （P〈0.05）. Compared with the model group, the serum levels of IL-4, IgE and sIgE in allergic rhinitis rats significantly decreased （P 〈0.05）, and the difference was not statistically significant （P 〉 0.05）. Conclusion Artemisia volatile oil had obvious effects on the anti-inflammatory and anti-allergic.
作者
徐云
刘燕
史玉柱
杨巧丽
黄华
顾政一
XU Yun LIU Yan SHI Yuzhu YANG Qiaoli HUANG Hua GU Zhengyi(College of Pharmacy ,Xinjing Medicial University, Urumqi 830011, China The Xinjiang Institute of Materia Medic, Urumqi 830004, China)
出处
《新疆医科大学学报》
CAS
2017年第6期805-809,共5页
Journal of Xinjiang Medical University
基金
国家自然科学基金(U1303224)
关键词
一枝蒿挥发油
抗炎
抗过敏
Artemisia volatile oil
Anti-inflammatory
Anti-allergic
