摘要
目的:观察化瘀解毒中药对梗阻性肾病大鼠肾脏类肝素酶(Hpa)表达的影响,以探索其拮抗炎症损伤的机制。方法:采用单侧输尿管结扎手术复制梗阻性肾病模型;采用real-time PCR技术检测肾组织Hpa mRNA的表达;采用SABC法检测肾组织Hpa蛋白的表达。结果:与假手术组比较,模型组大鼠肾组织Hpa mRNA及蛋白表达上调(P<0.01);与模型组比较,中药组肾组织Hpa表达下降(P<0.01)。结论:化瘀解毒中药可下调梗阻性肾病大鼠肾脏Hpa表达从而减轻炎症损伤。
Objective： to observe the effect of stasis-resolving and toxin-removing Chinese medicine on heparanases （ Hpa） expres-sion in obstructive nephropathy rats so to explore its mechanism of anti-inflammatory injury. Methods ： Take unilateral ureteral obstruc-tion to duplicate obstructive nephropathy models; take real-time technique to detect Hpa mRNA expression; take SABC to detect Hpa protein expression. Results： compared to sham operation group, Hpa mRNA expression of model group increased （ P 〈0. 01） ; com-pared to model group, Hpa expression of medicine group decreased （ P 〈 0. 01 ） . Conclusion： Stasis-resolving and toxin-removing Chinese medicine can decrease the Hpa expression in obstructive nephropathy rats and so as to lighten the inflammatory injury.
出处
《河北中医药学报》
2017年第2期5-7,共3页
Journal of Hebei Traditional Chinese Medicine and Pharmacology
基金
河北省科技计划项目:No.152777208
关键词
化瘀解毒中药
类肝素酶
梗阻性肾病
炎症损伤
stasis-resolving and toxin-removing Chinese medicine
heparanases （Hpa）
obstructive nephropathy
inflammatory injury
