摘要
目的建立测定药材腊梅花中芦丁含量的反相高效液相色谱(RP-HPLC)法。方法色谱柱采用Agilent C18柱(250 mm×4.6 mm,5μm),以甲醇-0.05%磷酸水溶液(35∶65)为流动相,流速为0.8 m L/min,柱温为30℃。结果以芦丁峰面积的常用对数(Y)对进样量的常用对数(X)进行线性回归,得回归方程Y=1 643 977.49 X+34 610.54,线性范围为0.005 19~1.557 0μg(r2=1.00),加样回收率为98.91%,RSD为1.55%(n=6)。结论该方法简便、准确,重复性好,可用于腊梅花的质量控制。
Objective To establish an RP-HPLC method for the content determination of medicinal flowers Lamei Rutin. Methods Agilent C18 column（250 mm × 4. 6 mm, 5 μm） was used, the column temperature was 30 ℃, Methanol-0. 05% phosphoric acid aqueous solution （ 35：65 ） , the flow rate was 0. 8 mL/min. Results In the selected chromatographic conditions, the peak area of the common logarithm （ Y ） of the commonly used sample size to number of （ X ） for linear regression, the regression equation was Y=1643977. 49 X＋34610. 54, the linear range was 0. 00519-1. 5570 μg （ r^2=1. 00 ） , the recovery rate was 98. 91%, RSD was 1. 55%（ n=6 ） . Conclusion This method is simple, accurate, reproducible, which can be used for the quality control of Rutin Chimonanthus flowers.
出处
《中国药业》
CAS
2017年第3期30-32,共3页
China Pharmaceuticals
