摘要
目的了解泌尿系感染常见细菌对尿液中磷酸铵镁结晶形成的影响,为临床积极抗感染治疗,防止结石形成提供依据。方法将大肠埃希菌、奇异变形杆菌、铜绿假单胞菌、肺炎克雷伯菌、肠球菌五种细菌分别配成0.5McFarland,取10μl菌悬液于2 ml新鲜无细菌及其他有形成分的尿液中,置35℃孵箱培养,24 h和48 h后用超高倍显微镜对培养液进行观察和对磷酸铵镁结晶计数。25μl菌悬液于5 ml新鲜无细菌及其他有形成分的尿液重复进行试验。结果从无磷酸铵镁结晶培养液观察到磷酸铵镁结晶,男性形成率高于女性。奇异变形杆菌培养液中磷酸铵镁结晶形成最多,铜绿假单胞菌次之,肺炎克雷伯菌第三,肠球菌在2 ml培养液有1例形成,大肠埃希菌在5 ml的培养液中有2例形成。形成的结晶以未成形羽毛状结晶最多,立方形和方柱状次之,洋信封状结晶最少。结论具有尿素酶细菌在尿液中繁殖时容易促进磷酸铵镁结晶形成,奇异变形杆菌是促进磷酸铵镁结晶形成的重要病原菌之一。
Objective To study formation of ammonion-magnesium phosphate crystals in urine with bacteria growing and pro- vide guidance for cilinical prevention of urinary calculi. Methods Bacterial culturefluid of Escherichia coil,Proteus mirabi- lis, Pseudomonas aeruginosa ,Klevsiella pneumoniae ,Enterococcus in urine was examined directly under the ultrahigh sensi- tive microscpcope system for ammonion-magnesium phosphate crystasl. The number of ammonion-magnesium phosphate crystasl was measured when the 24th and the 48th hour. Results Ammonion-magnesium phosphate crystasl were observed from the culture fluid without ammonion-magnesium phosphate crystasl. The rate of male formation was higher than that of female. Ammonion-magnesium phosphate crystals in culture fluid of Proteus mirabilis was the highest, Pseudomonas aerugi- nosa was the second,the third was Klebsiella pneumoniae ,and there was formed 1 case in 2 ml culturefluid of enterococcus, and 2 cases of formation in 5 ml culturefluid of Escherichia coll. The crystals formed were the most unformed feather crys- tals,followed by cubic and square cylinders,and the envelope-like crystals were the least. Conclusion Bacteria with urease play a significant role in ammonion-magnesium phosphate crystasl formed,Proteus mirabilis is the main pathlogen.
出处
《现代检验医学杂志》
CAS
2017年第2期131-134,共4页
Journal of Modern Laboratory Medicine
关键词
磷酸铵镁结晶
细菌
培养
尿液
ammonion-magnesium phosphate crystals
bacteria
culture
urine
