摘要
目的探讨荜茇酰胺(PL)对人胆管癌HCCC-9810细胞增殖、凋亡和生长迁移的影响。方法用MTT法检测不同浓度(0、5、20μmol/L)的PL干预后的人胆管癌HCCC-9810细胞增殖活力,并用Hochest33258试验和细胞划痕试验分别检测HCCC-9810细胞的凋亡和生长迁移情况。结果人胆管癌HCCC-9810细胞经不同浓度(0、5、20μmol/L)的PL处理后,其PL浓度越高,细胞增值率越降低(P<0.01),核变形细胞比例越高(P<0.01)。结论 PL能明显抑制人胆管癌HCCC-9810细胞的增殖,且具有明显的浓度依赖性;PL且可诱导人胆管癌HCCC-9810细胞的凋亡,并明显抑制人胆管癌HCCC-9810细胞生长迁移。
Objective To investigate the effect of piperlongumine（PL） on the proliferation, apoptosis, growth and migration of human cholangiocarcinoma hccc-9810 cells. Methods MTT assay was used to detect the proliferation activity of human cholangiocarcinoma hccc-9810 cells after intervention with the PL of different concentration（0, 5 and 20 μmol/L）, and Hochest33258 test and cell scratch test were used to detect the apoptosis, growth and migration of human cholangiocarcinoma hccc-9810 cells, respectively. Results After human cholangiocarcinoma hccc-9810 cells were treated with the PL of different concentration（0, 5 and 20 μmol/L）, the higher the PL concentration, the lower the cell proliferation rate（P〈0.01）, and the higher the percentage of the cells with deformed nuclear（P〈0.01）. Conclusion PL can inhibit the proliferation of human cholangiocarcinoma hccc-9810 cells and has significant concentration dependence; PL may induce the apoptosis of human cholangiocarcinoma hccc-9810 cells and significantly inhibit the growth and migration of human cholangiocarcinoma hccc-9810 cells.
出处
《广东医学院学报》
2016年第5期484-486,490,共4页
Journal of Guangdong Medical College
基金
广东省中医药局建设中医药强省立项资助科研课题(No.20141297)
