摘要
目的:利用高场不对称波形离子迁移谱(FAIMS)技术检测并识别常见伤口感染细菌。方法:在合适的实验条件下,用FAIMS分析仪检测大肠杆菌(ECO)、金黄色葡萄球菌(SAU)、铜绿假单胞菌(PAE)的TH肉汤培养液及纯TH肉汤培养液4种样品,得到其离子迁移谱图。分析谱图形状,提取峰值点,通过对比分析确定3种细菌TH培养液的特征离子峰。结果:ECO与SAU均有有别于另外3种样品的稳定特征离子峰,特征峰可用场强、补偿电压、离子电流值3个参量确定。PAE出现两个特征峰,其中一个不稳定出现,但依然可凭借第二个特征峰进行辨别。结论:FAIMS技术可对ECO、SAU、PAE这3种常见细菌的TH肉汤细菌培养液进行甄别,为伤口感染的快速检测提供一定的可行性。
Objective To detect and identify common wound-infecting bacterial species using high-field asymmetric ion mobility spectrometry（FAIMS）. Methods Under optimal experimental conditions, escherichia coli（ECO）, staphylococcus aureus（SAU）, pseudomonas aeruginosa（PAE） cultured in thioglycolate（TH） broth and bare TH broth were tested by FAIMS analyzer,and their ion mobility spectra were obtained. The spectra shape was analyzed and the peaks were extracted. The characteristic ion peaks of the three kinds of bacteria in TH broth were determined by comparative analysis. Resulsts Both ECO and SAU showed stable characteristic ion peaks that differentiate them from the other three samples. The characteristic peaks can be defined by dispersion field, compensation voltage and ion current. PAE showed two characteristic ion peaks, with an unstable one and the other which was stable enough for the identification of the bacterium. Conclusion FAIMS can differentiate ECO, SAU and PAE cultured in TH broth, which are the three most common kinds of bacteria found in infected wound,suggesting a new method for the rapid identification of wound-infecting bacteria.
出处
《中国医学物理学杂志》
CSCD
2017年第3期297-301,共5页
Chinese Journal of Medical Physics
基金
国家国际科技合作专项(2014DFA31560)
重庆市科技研发基地建设计划(国际科技合作)(cstc2013gjhz10003)
关键词
高场不对称波形离子迁移谱
细菌检测
特征离子峰
大肠杆菌
金黄色葡萄球菌
铜绿假单胞菌
high-field asymmetric ion mobility spectrometry
bacterium detection
characteristic ion peak
escherichia coli
staphylococcus aureus
pseudomonas aeruginosa
