摘要
A new cross-linked heptapeptide matrix(CHM)was prepared and the adsorption performance for papain was investigated.Firstly,a phage-displayed heptapeptide(Ile-Gln-SerPro-His-Phe-Phe)with high affinity to papain was found.Secondly,the heptapeptide was cross-linked to a matrix for papain adsorption.Finally,the adsorption conditions were optimized by response surface methodology.The results indicated that the optimum adsorptionconditionsweredeterminedasinitialpH 6.9,temperature 40℃,and the adsorption capacity of CHM for papain was found to be 55.52 mg/g.Moreover,the papain was purified 78-fold in a single step determined by affinity precipitation.More than87%of the adsorbed papain was desorbed using the eluent solventtriethylamine at pH 7.4.The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.
A new cross-linked heptapeptide matrix （CHM） was prepared and the adsorption performance for papain was investigated. Firstly, a phage-displayed heptapeptide （lle-Gin.Ser- Pro-His.Phe-Phe） with high affinity to papain was found. Secondly, the heptapeptide was cross-linked to a matrix for papain adsorption. Finally, the adsorption conditions were optimized by response surface methodology. The results indicated that the optimum adsorption conditions were determined as initial pH 6.9, temperature 40℃, and the adsorption capacity of CHM for papain was found to be 55.52 mg/g. Moreover, the papain was purified 78- fold in a single step determined by affinity precipitation. More than 87 % of the adsorbed papain was desorbed using the eluent solventtriethylarnine at pH 7.4. The results show that the CHM is a promising adsorbent for papain purification from crude papaya powder.
基金
Fundamental Research Funds for the Central Universities,China(No.2232015D3-15)
Shanghai Natural Science Foundation,China(No.14ZR1401300)
Shanghai Pujiang Program,China(No.16PJD007)
“111 Project”Biomedical Textile Materials Science and Technology,China(No.B07024)
