摘要
目的检测特发性膜性肾病（IMN）患者血、尿、肾组织中氧化应激相关因子超氧化物歧化酶（SOD）、血红素氧合酶1（HO-1）、丙二醛（MDA）、8-羟基脱氧鸟苷（8-OHdG）的水平及表达，探讨氧化应激参与IMN发生的可能机制。方法收集2016年1月至2016年6月郑州大学第一附属医院肾内科经。肾穿刺病理检查诊断的IMN（40例）、继发性膜性肾病（SMN，20例）、微小病变型。肾病（MCD，20例）及健康对照（NC，20例）的血、尿和肾组织标本。用免疫组织化学法检测肾组织SOD、HO-1、8-OHdG的表达情况；ELISA法检测血、尿标本中SOD、HO-1、MDA、8-OHdG水平，并探讨其与尿蛋白／肌酐（uPCR）的相关性。结果IMN组患者肾组织SOD、HO-1及8-OHdG的相对表达量和血清SOD水平明显高于MCD组及NC组（均P〈0．01）；IMN组患者血清和尿液中HO-1、MDA及8-OHdG水平亦高于MCD及NC组（均P〈0．05）。IMN组患者尿8-OHdG及MDA水平与尿蛋白／肌酐（uPCR）呈正相关（均P〈0．05）。结论IMN患者氧化应激相关因子表达升高，且与临床密切相关，证实了氧化应激参与IMN的发生。
Objective To investigate the expression of oxidative stress related factors, such as, supcroxide dismutase （SOD）, heme oxygenase-1 （HO-1）, malondialdehyde （MDA） and 8-hydroxy-2- deoxyguanosine （8-OHdG） in patients with idiopathic membranous nephropathy （IMN） and explore the possible role of oxidative stress in the pathogenesis of IMN. Methods Serum, urine and renal tissue of 40 patients with IMN, 20 patients with secondary membranous nephropathy （SMN）, and 20 patients with minimal change nephropathy （MCD） were collected from the nephrology department of the First Affiliated Hospital of Zhengzhou University. All of them were diagnosed by renal biopsy from January 2016 to June 2016. Serum, urine and renal tissue of 20 healthy persons were collected as the normal control （NC）. The expressions of SOD, HO- 1, and 8- OHdG in renal tissues were detected by immunohistochemistry. The levels of SOD, HO- 1, MDA and 8- OHdG in serum and urine specimens were detected by ELISA. The relationship between oxidative stress related factors and proteinuria in IMN was investigated as well. Results The expressions of SOD, HO - 1 and 8 - OHdG were significantly higher than those in MCD group and NC group in renal tissue; the expression of SOD in IMN group was significantly higher than that in MCD group and NC group in serum （all P 〈 0.01）. The expressions of HO-1, MDA and 8-OHdG in the serum and urine of the IMN group were significantly higher than those of the MCD group and the NC group （all P 〈 0.05）. There was a significant positive correlation between the urine expression of 8- OHdG and MDA and proteinuria in the IMN group. Conclusions Oxidative stress-related factors in IMN patients are elevated, which is closely related to clinical manifestations. In that sense, it is confirmed that oxidative stress is involved in the pathogenesis of IMN.
出处
《中华肾脏病杂志》
CAS
CSCD
北大核心
2017年第3期175-179,共5页
Chinese Journal of Nephrology
基金
十三五国家重点研发计划重大慢性非传染性疾病防控研究（2016YFC1305404）
河南省自然科学基金项目（162300410282）
河南省医学科技攻关计划项目（201503043）
