摘要
应用科赫氏法则测定了从兰屿肉桂炭疽病病样中分离的纯培养物的致病性,对其进行鉴定和生物学特性研究。结果表明:兰屿肉桂炭疽病的病原菌为Colletotrichum aotearoa B.Weir&P.R.Johnst,可侵染8科11种供试寄主植物。培养基对病原菌菌丝生长、产孢量和孢子萌发的影响差异显著,菌丝生长最佳培养基为PDA,产孢最佳培养基为PYG和CMA,孢子萌发最佳培养基为CMA。碳氮源对病原菌菌丝生长、产孢量和孢子萌发的影响差异明显,菌丝生长最佳碳氮源为蔗糖和蛋白胨,产孢最佳碳氮源为果糖和蛋白胨,孢子萌发最佳碳氮源为肌醇和酵母浸膏。病原菌菌丝生长、产孢和孢子萌发的最佳温度和p H值分别为25℃和7.0。光照抑制病原菌菌丝生长和孢子产生,相对湿度高有利于孢子萌发,高于98%时,萌发率达100%。
To confirm the pathogen of anthracnose diseases on Cinnamomum kotoense, the pathogenicity of isolates from diseased tissues was tested according to Koch＇s postulates. The isolate was tentatively identified using morphological characteristics and ITS sequence analysis. The biological characteristics were determined by the cultivation of Cinnamomum kotoense at different medium, carbon and nitrogen resources, temperatures, pH, light treatments and relative humidity. The results showed anthracnose diseases on Cinnamomum kotoense was caused by Colletotrichum aotearoa B. Weir ＆ P.R. Johnst, which can infect the eleven plants of eight families. Effects of different medium on myeelial growth, sporulation and spore germination were significant, optimal media for myeelial growth was PDA, for sporulation were PYG and CMA, and for spore germination was CMA. Effects of carbon and nitrogen sources on mycelial growth, sporulation spore germination were significant, optimal carbon and nitrogen source for myeelial growth were sucrose and peptone respectively, for sporulation were fructose and peptone, and for spore germination were inositol and yeast extract. The optimal temperature and pH for mycelial growth, sporulation and spore germination were 25 ~C and pH 7.0. Different light treatments affect the mycelial growth, sporulation and spore germination of Cinnamomum kotoense, light could inhibit on mycelial growth and sporulation. High relative humidity （RH） was advantageous to spore germination, when RH was higher than 98%, the percentage of spore germination was up to 100%.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2017年第3期24-31,共8页
Journal of Central South University of Forestry & Technology
基金
广东省科技计划项目(20090215)
