摘要
利用RAPD和SSR分子标记对粗皮桉7个种源遗传多样性进行分析,10条RAPD引物共扩增出157个位点,其中多态位点145个,多态性百分率92.34%,遗传一致度范围0.906 1~0.971 4;21对SSR引物共检测到252个等位变异,平均等位基因数为12个,平均有效等位基因数为4.6个,遗传一致度范围0.727 1~0.908 0。2种标记遗传一致度相关分析表明呈显著相关,说明2种标记分析粗皮桉遗传多样性具有较高一致性。聚类分析显示,RAPD标记将粗皮桉7个种源被划分为4个类群,SSR标记将粗皮桉7个种源被划分为3个类群,SSR标记聚类结果与粗皮桉种源地理分布密切相关。RAPD和SSR分子标记均适合粗皮桉遗传多样性分析,SSR标记更具可靠性。
RAPD and SSR markers were used to assess the genetic diversity of introduced Eucalyptus pelli- ta that were from 7 provenances. Ten RAPD primers were selected,157 bands were amplified,of which 145 bands （92. 34%） were polymorphic, and the Nei＇s unbiased measures of genetic identity ranged from 0. 906 1 to 0. 971 4. Two hundred and fifty two alleles were detected by 21 pairs of SSR primers and 12 alleles on average. The average effective number of alleles was 4.6,and the Nei＇s unbiased measures of genetic identity ranged from 0. 727 1 to 0. 908 0. The correlation of the Nei＇s unbiased measures of genetic identity between RAPD and SSR were significant,indicating that the two methods had high consistency. According to the UPGMA method, 7 E. pellita populations could be divided into four groups by RAPD, three groups by SSR,and the geographical distribution of E. pellita was closely related to the cluster result by SSR. Two molecular markers were feasible in the genetic diversity analysis of E. pellita. Further more, the SSR technique was more reliable.
出处
《西北林学院学报》
CSCD
北大核心
2017年第1期131-136,共6页
Journal of Northwest Forestry University
基金
广西林业科技项目(桂林科字2012-11)
广西林业科技项目(桂林科字[2013]第1号)
广西优良用材林资源培育重点实验室开放课题(12A0101)
