摘要
【目的】通过ISSR分子标记技术,分析宝华玉兰种质资源的遗传多样性,为该珍稀濒危植物的利用和保护提供理论依据和技术支持。【方法】利用ISSR分子标记对江苏句容宝华山的20个宝华玉兰单株进行遗传多样性分析。【结果】用筛选获得的6条引物对样品进行PCR扩增,共扩增出50个清晰的扩增位点,其中多态性位点34个,多态性位点比率为（PPB）68%。通过POPGENE32软件计算出有效等位基因数（Ne）、Nei＇s基因多样性指数（H）和Shannon信息多样性指数（I）分别为1.406 8、0.235 6、0.351 2。利用NTSYSpc-2.1软件进行UPGMA聚类,各个样品间的遗传相似系数在0.58～1.0。【结论】宝华玉兰具有丰富的遗传多样性,20个“生单株间的遗传相似度较高,亲缘关系较近。
[Objective] The aim of the study was to assess the genetic diversity of Magnolia zenii by using inter-simple sequence repeat （ISSR）markers technique. The results could provide a theoretical basis and technical support for the use and protection of this rare and endangered species. [Methods] ISSR markers was used to assess the genetic diversity of 20 Magnolia zenii individuals. [Results] Six primers were selected and used for PCR amplification. 50 discernible DNA fragments were produced, of these, 34 were polymorphic loci and the percentage of polymorphic loci PPB was 68%. Based on POPGENE32, the effective number of alleles （Ne）, Nei＇s gene diversity （H） and Shannon＇s Information index （I） were 1.406 8, 0.235 6 and 0.351 2, respectively. According to cluster analysis with NTSYSpc-2.1, genetic similarity coefficient between each sample ranged from 0.58 to 1.0. [Conclusion] There is abundant genetic diversity of Magnolia zenii and there is high genetic similarity and close genetic relationship among the 20 individuals.
作者
陈云霞
南程慧
CHEN Yun-xia NAN Cheng-hui(Nanjing Forest Police College, Nanjing 210023, China)
出处
《四川农业大学学报》
CSCD
北大核心
2016年第4期445-449,共5页
Journal of Sichuan Agricultural University
基金
中央高校基本科研业务费专项资金项目(LGZD201505)
