摘要
目的 探讨福州地区献血者人群隐匿性乙型肝炎病毒感染（OBI）的流行病学、血清学表现以及氨基酸突变情况。方法 应用血清学检测与核酸扩增检测技术（NAT）对福州地区102 866例献血者标本做ELISA HBsAg筛查及HBV DNA检测,排除乙肝两对半检测为阴性结果的标本,确定HBsAg（-）、HBV DNA（＋）为OBI标本;应用实时荧光定量PCR技术对OBI献血者和HBV阳性献血者标本做HBV DNA检测,采用巢式PCR扩增S区基因并测定序列,使用MEGA5.0软件对HBV基因分型和S区氨基酸做突变分析。结果共筛查出66例OBI标本,OBI率0.064%（66/102866）,其中抗-HBc阳性比例93.94%（62/66）,抗-HBs阳性率22.72%;HBV DNA为13-302IU/mL。男性献血者OBI检出率高于女性（P〈0.05）,36-45岁组献血者OBI检出率最高,18-25岁组检出率最低;比较ABO 4种血型献血者OBI检出率的差异无统计学意义（P〉0.05）。10例OBI献血者B型7例、C型3例。OBI献血者HBsAg“a”表位氨基酸突变位点主要集中在aa131-134和aa143-144,其中131、133、134和143、144位点具有较高的突变率。结论 福州地区献血者人群存在一定比例OBI,OBI献血者具有一定的流行特征,HBsAg“a”表位氨基酸突变位点主要集中在aa131-134和aa143-144。
Objective To investigate the epidemiology, serology of the occult hepatitis B virus infection （OBI） in blood donors in Fuzhou, and the mutation of amino-acid sequence in genome. Methods A total of 102 866 samples were collected from blood donors in Fuzhou. All samples were tested by Serologic testing of HBsAg and nucleic acid testing, Then the HBsAg- HBV DNA＋ samples were tested by serologic screening for HBV antigen and antibody. The OBI samples were performed by real-time fluorescent quantitative PCR detection, nest-PCR on S region of viral genome, then the genotype and the mutation of the viral strains were analyzed based on the sequences. Results Sixty-six of 102 866 （0. 064%） samples were selected as OBI samples. Sixty-two of 66 （93.9%） OBI samples were anti-HBc positive, only 22.72% samples could detected HBV DNA. The DNA copies were between 13 to 302 IU/mL. The OBI detection rate in male donors was significantly higher than female （P 〈0.05）. The detection rate of OBI was the highest among the age group of 36-45 years old and the lowest was OBI of 18-25 years old group. There was no significant difference among the donors of different blood types. 10 samples could be amplified with nest-PCR, 7 was B genotype, and 3 was C genotype. The mutation sites in OBI donors were mainly located in aa131-134 and aa143-144, and the frequency of mutations in 131, 133, 134 and 143, 144 loci were more frequent. Conclusion The prevalence of OBI in blood donors in Fuzhou is 0. 064% . OBI donors have certain epidemiological characteristics. The amino acid mutation sites of HBsAg ＂ a＂ epitopes are mainly located in aa131-134 and aa143-144.
出处
《福建医药杂志》
CAS
2016年第6期85-89,共5页
Fujian Medical Journal
基金
福建省卫生厅青年科研课题(2012-1-13)
