摘要
目的:通过观察大鼠免疫及瘀血状态的变化从而研究黄芪桂枝五物汤对阳虚寒凝型骨关节炎大鼠的影响。方法:50只大鼠随机分为正常组,模型组,塞来昔布组(20.82 mg·kg^(-1)),黄芪桂枝五物汤7 d组和黄芪桂枝五物汤14 d组(30 g·kg^(-1))。除正常组外,各造模组大鼠分别采用冷固法加游泳、隔日给食及寒冷环境刺激的方法复制阳虚寒凝型骨关节炎模型。造模结束前最后2周,各给药组分别按6 m L·kg^(-1)给药,黄芪桂枝五物汤14 d组ig给药14 d,其余各给药组分别ig 7 d,正常组及模型组予生理盐水ig 7 d。ig结束后,分别观察各组大鼠一般状态;量取膝关节周长,计算膝关节肿胀度;苏木素-伊红(HE)染色观察大鼠下肢膝骨关节的病理变化;取大鼠血液,酶联免疫吸附测定(ELISA)检测血清液肿瘤坏死因子-α(TNF-α),白细胞介素^(-1)β(IL^(-1)β),血浆前列腺素E2(PGE2),血栓素B2(TXB2),6-酮前列腺素F1α(6-keto-PGF1α)含量,生化法检测血清超氧化物歧化酶(SOD)水平。结果:与正常组比较,模型组大鼠关机肿胀度明显升高,胶原纤维增粗、血管翳及炎性细胞浸润较为明显,大鼠血清TNF-α,IL^(-1)β,SOD及6-keto-PGF1α水平明显降低,PGE2,TXB2水平,TXB2/6-keto-PGF1α明显升高(P<0.05,P<0.01);与模型组比较,黄芪桂枝五物汤使造模后大鼠毛色变得柔亮,尾部瘀痕减轻,双下肢活动轻微受限,减少大鼠跛行;关节肿胀度降低,胶原纤维增粗、血管翳及炎性细胞浸润程度减轻,黄芪桂枝五物汤给药后使TNF-α,IL^(-1)β,SOD及6-keto-PGF1α水平升高(P<0.05),PGE2,TXB2水平,TXB2/6-keto-PGF1α降低(P<0.05)。结论:黄芪桂枝五物汤对阳虚寒凝型骨关节炎疗效确切,黄芪桂枝五物汤的作用效果与用药时间有关,其作用机制与抗炎及改善机体瘀血状态有关,并有可能通过抗炎细胞因子的调节作用影响机体瘀血状态。
Objective： To study the effect of Huangqi Guizhi Wuwutang on immunology and blood stasis state of osteoarthritis rats with Yang deficiency and cold coagulation symptoms. Method： Totally 50 rats were randomly divided into normal group,model group,celecoxib group（ 20. 82 mg·kg-1）,Huangqi Guizhi Wuwu Tang 7 d group and Huangqi Guizhi Wuwu Tang 14 d group（ 30 g·kg-1）. Except for the normal group,all of the other groups were included in the osteoarthritis model with Yang deficiency and cold coagulation symptoms through cold fix method plus swimming,feeding every two days and cool environment. During the last two weeks before successful modeling,all groups were administered with drugs at dose of 6 m L·kg-1. Huangqi Guizhi Wuwu Tang 14 d group was administered with Huangqi Guizhi Wuwu Tang for 14 days,other groups were given respective drugs for7 days; meanwhile,normal group and model group were given insaline for 7 days. After intragastric administration,the general state of all of the groups was observed. Efforts were made to calculate the perimeter and swollen of knees.HE was performed to observed pathological changes in knee joint. Tumor necrosis factor-α（ TNF-α）,interleukins-1β（ IL-1β）,plasma prostaglandin E2（ PGE2）,thromboxane B2（ TXB2）,6-keto-PGF1αwere tested by ELISA,and superoxide dismutase（ SOD） was tested by biochemical method. Result： Compared with normal group,model group showed significant increase in joint swelling,PGE2,TXB2,TXB2/6-PGF1αcontent in serum,obvious enlargement of collagen fibers,pannus and inflammatory cell infiltration,and significant decrease in TNF-α,IL-1β,SOD and 6-keto-PGF1α（ P 〈0. 05,P 〈0. 01）. Compared with model group,Huangqi Guizhi Wuwu Tang made the fur smooth and the reduction in tail bruise. Limbers were slightly limited,claudication,swelling of knee joints,collagen fiber,pannus and inflammatory cells in Huangqi Guizhi Wuwu Tang groups were lower than model group. Meanwhile,Huangqi Guizhi Wuwu Tang can significantly increase the content of TNF-α,IL-1β,SOD and 6-keto-PGF1α（ P 〈0. 05）,and decrease the content of PGE2,SOD,TXB2,TXB2/6-keto-PGF1α（ P 〈0. 05）. Conclusion： Huangqi Guizhi Wuwu Tang has an obvious effect in treating osteoarthritis with Yang deficiency and cold coagulation symptoms,which is correlated with the medication time. Its mechanism is correlated with anti-inflammation and relieve of blood stasis state by regulating anti-inflammatory cytokines.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第23期149-154,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山西省科技厅攻关项目(2014011047-1)
