摘要
目的对GB/T 5009.181-2003和GB/T 8937-2006中食用动植物油脂中丙二醛的测定方法进行优化。方法样品采用三氯乙酸混合溶液经50℃恒温水浴震荡提取,与硫代巴比妥酸（thiobarbituric acid,TBA）作用生成粉红色化合物,在532 nm处采用分光光度计法测定。结果丙二醛在0-2.5mg/L范围内线性关系良好,r=0.9995,方法检出限为0.1 mg/kg,回收率在88.0%-100.0%之间,日内精密度和日间精密度小于6.09%。结论该方法扩大了检测范围,操作简便准确,灵敏度高,可适用于动植物油脂中丙二醛的测定。
Objective To optimize the method of GB/T 5009.181-2003 and GB/T 8937-2006 fordetermination of propyldialdehyde in animal and vegetable oils.Methods Samples were extracted with trichloroacetic acid solvent at50℃ by constant temperature water bath oscillators,the filtered solution was derivated by thiobarbituric acid for spectrophotometric test at a wavelength of 532 nm.Results Propyldialdehyde had a good linearity in the range of0-2.5 mg/L with the correlation coefficient of 0.9995.The limit of detection was 0.1 mg/kg,and the recovery was in the range of 88.0%-100.0%with inter-day precision and within-day precision less than 6.09%.Conclusion The improved method expands the scope of tests,which is more simple,rapid,accurate,and suitable for the determination of propyldialdehyde in animal and vegetable oils.
出处
《食品安全质量检测学报》
CAS
2016年第9期3477-3480,共4页
Journal of Food Safety and Quality
基金
2012食品安全国家标准制修订计划项目(spaq-2012-66)
广西重大专项资金资助(桂科重14121003-4-3)~~
关键词
动物油脂
植物油脂
丙二醛
测定方法
优化
animal oil
vegetable oils
propyldialdehyde
determination method
optimization
