摘要
目的:建立同时测定淫羊藿饮片中朝藿定A、朝藿定A1、朝藿定B、朝藿定C、淫羊藿苷、宝藿苷Ⅰ6种成分的含量测定方法。方法:采用高效液相色谱法,色谱柱为Agilent ZORBAX Eclipse XDB-C_(18)(150 mm×4.6 mm,5μm),流速为1.0m L·min^(-1),流动相为乙腈-水梯度洗脱,检测波长268 nm,柱温30℃。结果:6种成分均达到基线分离,各成分均有较宽的线性范围和良好的线性关系(r>0.999 7),回收率为97.8%~101.5%。结论:本方法准确、灵敏、可靠,重现性较好,应建立多种黄酮类成分作为测定指标,以全面控制淫羊藿药材的整体质量。
Objective： To determine six main flavonoids（ Icariin,Epimedin A1,Epimedin A,Epimedin B,Epimedin C,BaohuosideⅠ） simultaneously in herba epimedii by HPLC. Methods： The samples were separated on Agilent ZORBAX Eclipse XDB-C18（ 150 mm × 4. 6 mm,5 μm） with mobile phase consisted of acetonitrile- water（ gradient elution）. The detection wavelength was 268 nm and the column temperature was 30 ℃. Results： All the six flavonoids reached baseline separation with good linearity（ r〉 0. 999 7）. The average recovery rate rang 97. 8%- 101. 5%. Conclusion： The method is reliable,stable and well repeatable. We should establish multiple indicators of flavonoids instead of considering the index of Icariin only to achieve a full control on the quality of herba epimdii.
出处
《中医学报》
CAS
2016年第10期1559-1562,共4页
Acta Chinese Medicine
基金
济南军区后勤科研计划项目(CJN10L067
CJN14R062)
