摘要
目的:建立Ph^+急性淋巴细胞白血病(ALL)小鼠模型,为Ph^+ALL研究提供工具。方法:用M ig190逆转录病毒转染BABL/c小鼠骨髓细胞产生CML样小鼠,分选CML样小鼠BCR-ABL^+B细胞,输注同系小鼠建立Ph^+ALL模型;应用流式细胞术鉴定免疫表型,RT-PCR和Western blot鉴定BCR-ABL转录和蛋白表达。结果:M ig190逆转录病毒转染后小鼠迅速发生CM L样病变,输注来自CM L样小鼠的BCR-ABL^+B细胞后受鼠均发生急性淋巴细胞白血病,免疫分型显示白血病细胞CD19^+;RT-PCR和Western Blot均检测到BCR-ABL,符合Ph^+ALL的免疫表型和分子生物学特征。结论:成功开发了一种新型Ph^+ALL小鼠模型的建立方法,能够为研究Ph^+ALL提供一有力工具。
Objective：To establish a mouse model of Ph acute lymphoblastic leukemia（ALL） for providing a valuable tool to facilitate the researches on Ph＋ ALL.Methods：CML-like mice were generated by transfection to bone marrow cells of BABL/c with Migl90 retrovirus.The Ph＋ ALL mouse model was established by infusion of sorted CML like mouse-derived BCR-ABL＋ B cells into the mice of same linege.Immonophenotypes,BCR-ABL transcription and expression of these leukemic cells were detected by flow cytometry,RT-PCR and Western blot respectively.Results：CML-like presentation appeared in the mice after Migl90 virus transfection.After infusion with sorted BCRABL＋ B cells,all the receipted mice eventually presented the clinical signs of acute leukemia.Flow cytometry analysis showed that these leukemic cells were positive for CD19＋;both RT-PCR and Western blot indicated that this mouse model is consistent with Ph＋ ALL phenotypecally and molecalarlly.Conclusion：A novel method to establish Ph＋ ALL mouse model has been developed successfully,and this model can provide useful tool for the study of Ph＋ ALL.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2016年第5期1324-1328,共5页
Journal of Experimental Hematology
基金
国家自然科学基金(81471580、81272206、81300399)
江苏省普通高校研究生科研创新计划(SJEE14-0199)
