摘要
为了确定中间球海胆Strongylocentrotus intermedius幼胆饲料中适宜的脂肪水平,采用摄食生长试验探讨了饲料中脂肪水平（3%、6%、9%、12%和15%）对海胆幼胆存活、生长、消化酶和热胁迫后抗氧化酶活力的影响,试验共进行96 d。结果表明：试验结束时,6%饲料脂肪水平组海胆增重率最高,显著高于15%脂肪组（P〈0.05）;随着饲料脂肪水平的升高,中间球海胆消化道中脂肪酶活力显著升高（P〈0.05）,淀粉酶活力显著下降（P〈0.05）,胃蛋白酶活力有下降趋势（P〉0.05）;饲料脂肪水平显著影响热胁迫后海胆体腔液中抗氧化酶活力,其中热胁迫2 h后,12%脂肪组超氧化物歧化酶（SOD）活力最高,显著高于15%脂肪组（P〈0.05）;热胁迫6 h后,海胆体腔液过氧化氢酶（CAT）活力最高值出现在6%脂肪组,且高于12%和15%脂肪组。研究表明,6%饲料脂肪水平时中间球海胆幼胆的生长速率最快,6%~12%饲料脂肪水平能够显著提高热胁迫后海胆的抗氧化能力。
Juvenile sea urchin Strongylocentrotus intermedius were fed diets containing 3%,6%,9%,12% and15% of lipid for 96 days to investigate the effects of dietary lipid levels on growth performance,digestive enzyme activity and anti-oxidative capacity. Results showed that there was significantly higher or the maximal weight gain rate（ WGR） in the sea urchin fed the diet containing 3% lipid than that in the sea urchin fed the diet containing15% lipid（ P〈 0. 05）. The amylase activity was shown to be significantly lower（ P〈 0. 05） and lower in pepsin in the sea urchin juveniles fed the elevated lipid diets. Lipase activity was increased significantly with the increase in dietary lipid（ P〈 0. 05）. Relatively high dietary lipid（ 6%-12%） led to improve activity of superoxide dismutase（ SOD） and anti-oxidant capacity（ AOC） in the thermal stress sea urchin,with the maximal SOD activity in the sea urchin exposed to 2 and 6 hour thermal stress in the 12% dietary lipid group,and significantly higher than that in the 15% dietary lipid group（ P〈 0. 05）. The maximal catalase（ CAT） activity in coelomic fluid was observed in the sea urchin exposed to 15 min and 6 hour thermal stress in the 6% dietary lipid group,significantly higher than that in the 12% and 15% dietary lipid groups,and higher than that in the 15% dietary lipid group（ P〈 0. 05）,indicating that 6%-12% lipid was of the optimal for improvement of anti-oxidative capacity in response to thermal stress,though 6% lipid was suggested to be optimal for the growth of sea urchin.
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2016年第5期538-543,共6页
Journal of Dalian Ocean University
基金
辽宁省农业攻关及成果产业化项目(2015203003)
中国科协"青年人才托举工程"资助
国家自然科学基金资助项目(41606180)
关键词
中间球海胆
饲料脂肪水平
消化酶
抗氧化酶
Strongylocentrotus intermedius
dietary lipid level
digestive enzyme
anti-oxidative enzyme
