摘要
目的探索三虫半夏白术天麻汤治疗偏头痛的作用机制。方法建立硝酸甘油致大鼠偏头痛模型,给予半夏白术天麻汤高、中、低剂量灌胃干预7 d,以苯甲酸利扎曲普坦为西药阳性对照药物,并予空白组、模型组进行对照。运用RT-PCR检测5羟色胺1B、1D(5-HT1B/1D)受体的基因表达。结果 1)对5-HT1B受体表达的影响:与空白组相比,模型组显著低于空白组(P<0.01);中药低剂量组与空白组无显著差异(P>0.05),中药中剂量组、中药高剂量组、西药组及联合用药组显著低于空白组(P<0.01);中药低、中、高剂量组、西药组及联合用药组较模型组升高,但不具有显著差异(P>0.05)。2)对5-HT1D受体表达的影响:与空白组相比,中药中剂量组显著降低(P<0.05),其余各组,与空白组相比较无显著差异(P>0.05);与模型组相比,各组无显著差异(P>0.05);与中药中剂量组相比,空白组、中药低剂量组、西药组、联合用药组较中药中剂量组显著升高(P<0.05),中药高剂量组与中药中剂量组相比无明显差异(P>0.05)。结论 5-HT1B/1D受体调节异常为偏头痛的发病环节之一。三虫半夏白术天麻汤可以提高偏头痛大鼠脑组织5-HT1B受体的基因表达,对5-HT1D受体的基因表达具有双向调节作用,临床运用,剂量不宜过大。
Objective： To explore the mechanism of Sanchong Banxia Baizhu Tianma Decoction in the treatment of migraine. Methods： Migraine model of SD rats were set up with nitroglycerin,given Banxiabaizhutianma de- coction of high,medium and low-dose intervention respectively for 7 days,taking Rizatriptan Monobenzoate as a positive control drug medicine, compared with the blank control group and the model group. RT-PCR was used to test the genetic expression of the 5-HT1B receptor and the 5-HT1D receptor. Results： The influence on the ex- pression of the 5-HT1B receptor： compared with the blank control group,the model group was significantly lower than the control group （P〈 0.01 ）;there was no significant difference between traditional Chinese medicine low- dose group and the blank group （P〉 0.05）;traditional Chinese medicine medium dose group,high dose group, western medicine group and the combination group were significantly lower than the blank control group （P 〈 0.01 ）;after creating the model and finishing the administration,the expression rose high in low,medium and high dose groups of traditional Chinese medicine,western medicine group and the combination group,compared with the model group,without significant difference （P〉 0.05）. The influence on the expression of 5-HT1D receptor： compared with the blank control group,traditional Chinese medicine medium dose group decreased significantly（P 〈 0.05）;other groups had no significant differences,compared with the control blank group （P〉 0.05）;compared with the model group,there was no significant difference among them （P〉 0.05）;compared with the traditional Chinese medicine medium dose group, the blank control group, traditional Chinese medicine low dose group ,west-eru medicine group and combination group were significantly higher （P 〈 0.05）;there was no difference between traditional Chinese medicine high-dose group and the traditional Chinese medium-dose group（P〉0.05）. Conclusion： 5-HT1B/1D receptor dysregulation is one cause of the pathogenesis of migraine. Sanchong banxia baizhu tianrna decoction can improve the genetic expression of 5-HT1B receptors in migraine rats＇ brains,and has a dual-direction regulation on genetic expression of the 5-HT1D receptors. In the field of clinical application,the dose should not be too large.
作者
陈少玫
韦启志
奚胜艳
李世勇
CHEN Shaomei WEI Qizhi XI Shengyan et al(Xiamen Hospital Affiliated to Fujian University of TCM, Fujian ,Xiamen 361009, China)
出处
《中国中医急症》
2016年第10期1874-1876,1880,共4页
Journal of Emergency in Traditional Chinese Medicine
基金
2012福建省中医药科研课题(wst201211)
关键词
5-HT1B/1D受体激动剂
利扎曲普坦
偏头痛
中医药
三虫半夏白术天麻汤
5-HT1B/1D receptor agonist
Rizatriptan Monobenzoate
Migraine
Traditional Chinese medicine
Sanchong Banxia Baizhu Tianma Decoction
