摘要
利用液体培养法研究模拟干旱胁迫环境(轻度胁迫、中度胁迫、重度胁迫)及不同难溶性磷酸盐对5株解无机磷细菌(P6、P7、P8、P19、P25)溶磷能力的影响。结果表明:菌株P6和P7对4种磷酸盐的溶解能力较佳,P6对磷酸铝、磷酸铁、磷酸氢钙及磷酸钙的溶磷量分别较CK(对照)显著提高143.7%、134.5%、1 318.3%、1 396.6%;P7对磷酸铁、磷酸氢钙及磷酸钙的溶磷量分别较CK显著提高348.0%、996.5%、1 810.4%。解无机磷细菌对磷酸钙的溶磷量与培养液p H值呈显著负相关(r=-0.920),与培养液有机酸质量浓度呈显著正相关(r=0.947);在3个梯度干旱胁迫下,P6、P7、P25解磷能力均显著低于CK,且随着胁迫程度的加深解磷能力呈现明显下降趋势;P8在轻度胁迫和中度胁迫下解磷能力均高于CK;P19在3个梯度干旱胁迫下解磷能力均高于CK。研究表明,5株解无机磷细菌对磷酸钙和磷酸氢钙的溶解效果较佳,对磷酸铁和磷酸铝的溶解效果较差;菌株P8、P19耐干旱能力较强,具有应用于干旱地区解磷的潜力。
The experiment was conducted to study the effects of drought stress （ mild stress, moderate stress, severe stress） and different insoluble phosphates on phosphate-solubilizing capacity of phosphate-solubilizing bacteria （P6, P7, P8, P19, P25） in liquid media. P6 and P7 had the higher solubilizing capacity of different insoluble phosphates, phosphate-solubi- lizing capacity of P6 in A1PO4, FePO4 · 4H20, CaHPO4 · 2H20 and Ca3（PO4）2 were 143.7%, 134.5%, 1318.3% and 1396.6%, significantly higher than those of CK, respectively. The phosphate-solubilizing capacity of P7 in FePO4 ·4H20, CaHPO4 · 2H20 and Ca3（PO4） 2 were 348.0%, 996.5% and 1810.4%, significantly higher than those of CK, respective- ly. The content of available phosphorous of Ca3（ PO4 ）2 had a significantly negative correlation with pH, and had a signifi- cantly positive correlation with total content of organic acids. Under three drought stress conditions, the phosphate-solubi- lizing capacity of P6, P7 and P25 were significantly lower than that of CK, and tended to decline with the increasing stress intensity. The phosphate-solubilizing capacity of P8 was higher than that of CK under mild and moderate stress conditions. The phosphate-solubilizing capacity of P19 was higher than that of CK under three stress conditions. Five phosphate-solubi- lizing bacteria had the higher solubilizing capacity of Ca3（ PO4 ）2 and CaHPO4 · 2H2O than AlPO4 and FePO4 ·4H2O. P8 and P19 had stronger drought resistant ability with the most promising bacteria for phosphate-solubilizing in arid area.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2016年第9期93-98,共6页
Journal of Northeast Forestry University
基金
教育部博士点学科专项基金(20123515110011)
福建省科技重大专项(2012NZ01)
关键词
桉树根
解无机磷细菌
磷源
有效磷
干旱胁迫
Eucalyptus rhizosphere
Phosphate-solubilizing bacteria
Phosphorus source
Available phosphorous
Drought stress
