摘要
目的比较金属基质蛋白酶-9(MMP-9)、基质金属蛋白酶抑制剂(TIMP-1)、新生血管因子(VEGF)在翼状胬肉中的表达,探讨3者在翼状胬肉中的作用。方法收集复发性翼状胬肉患者翼状胬肉标本38例、初发性翼状胬肉患者翼状胬肉标本47例和斜视患者球结膜标本32例。采用免疫组化法检测85例翼状胬肉和32例正常人球结膜中MMP-9、TIMP-1及VEGF的表达,比较MMP-9、TIMP-1及VEGF在3组中的表达差异,分析3者在翼状胬肉中表达的相关性。结果复发性翼状胬肉和初发翼状胬肉标本均有MMP-9、TIMP-1和VEGF的表达,MMP-9、TIMP-1和VEGF在复发翼状胬肉和初发翼状胬肉中阳性表达均显著高于正常球结膜。经相关检验分析,在翼状胬肉中,MMP-9和VEGF的阳性表达存在相关性;而TIMP-1与VEGF表达间无显著相关性。结论 MMP-9在翼状胬肉发生和复发过程中发挥重要作用。其可能通过破坏细胞外基质的屏障和促进VEGF产生来调控翼状胬肉的发生和复发。细胞外基质屏障的打破可能是翼状胬肉发病的基础。
Objective To investigate the expression of vascular endothelial growth factor （ VEGF）, matrix metallo- proteinase 9 （MMP-9） and matrix metalloproteinase inhibitor 1 （TIMP-1） in pterygium and find the roles of them in this disease. Methods Thirty-eight recurrent pterygium, forty-seven early onset pterygium and thirty-two normal bulbar conjunctivas were obtained in present study. The expressions of MMP-9, TIMP-1 and VEGF were tested in 85 cases of pterygium and 32 cases of normal eonjunctival by using immune histochemical method. The expressions of the MMP-9, TIMP-1 and VEGF were compared in the three groups of patients and the relations among them were analyzed. Results The MMP-9, TIMP-1 and VEGF were expressed in the recurrent pterygium and early pterygium specimens. The positive rates of MMP-9, TIMP-1 and VEGF significantly increased in the recurrent and early pte- rygium pterygium samples compared with the normal conjunctiva samples. In pterygium, there was a relation be- tween expression MMP-9 and VEGF correlation exists, but there was no correlation between TIMP-1 and VEGF by using the X2 analysis. Conclusion MMP-9 plays an important role in the pathogenosis of occurrence and recur- rence pterygium. It may destroy the extracellular matrix barriers and promote VEGF production to control the occur- rence and recurrence of pterygimn. Breaking down the extracellular matrix barriers may be the basis in human pterygium pathogenesis.
作者
李盈龙
马应
王李松
蒋正轩
Li Yinglong Ma Ying Wang Lisong et al(Dept of Ophthamology, The Thind Affiliated Hospital of Anhui Medical University, The First People＇s Hospital of Hefei, Hefei 230061)
出处
《安徽医科大学学报》
CAS
北大核心
2016年第10期1490-1494,共5页
Acta Universitatis Medicinalis Anhui
基金
安徽省高校省级自然科学研究项目(编号:KJ2013A147)
关键词
细胞外基质
翼状胬肉
复发
免疫组织化学
新生血管
extracellular matrix
pterygia
recurrent
immunohistochemieal
neovascularisation
